Relaxin-like factor and methods and uses thereof
View Patent ↗The present invention relates to a relaxin-like factor, its derivatives or analogs, and uses thereof. The present invention further relates to compositions comprising a relaxin-like factor, its derivatives or analogs, and relaxin wherein such composition exhibits an additive or synergistic effect.
1. A method of decreasing collagen synthesis, comprising:
administering a synthetic relaxin-like factor to cells of a human, wherein said cells express relaxin receptors; and
allowing the synthetic relaxin-like factor to contact the receptors for a period of time and under conditions such that the receptors are activated, and collagen synthesis is decreased;
the synthetic relaxin-like factor comprising an A chain and a B chain,
said A chain having the amino acid sequence:
Ala-Ala-Ala-Thr-Asn-Pro-Ala-Arg-Tyr-Cys-Cys-Leu-Ser-Gly-Cys-Thr-Gln-Gln-Asp-Leu-Leu-Thr-Leu-Cys-Pro-Tyr (SEQ ID NO:3)
or said amino acid sequence (SEQ ID NO:3) truncated by up to about 6 amino acids from the N terminus and/or by up to 6 amino acids from the C-terminus;
said B chain having the amino acid sequence:
Pro-Thr-Pro-Glu-Met-Arg-Glu-Lys-Leu-Cys-Gly-His-His-Phe-Val-Arg-Ala-Leu-Val-Arg-Val-Cys-Gly-Gly-Pro-Arg-Trp-Ser-Thr-Glu-Ala (SEQ ID NO:4)
or said amino acid sequence (SEQ ID NO:4) truncated by up to 5 amino acids from the N-terminus and/or by up to 5 amino acids from the C-terminus;
said A and B chains linked by disulfide bonds between amino acid residue number 11 of SEQ ID NO:3 and amino acid number 10 of SEQ ID NO:4.
2. The method of claim 1 , wherein the synthetic relaxin-like factor is attached to a detectable label.
3. The method of claim 1 , wherein the synthetic relaxin-like factor is chemically synthesized.
4. The method of claim 1 , wherein the synthetic relaxin-like factor is recombinantly produced.