Biopolymer marker indicative of disease state having a molecular weight of 2753 daltons
View Patent ↗The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of at least one disease state relative to recognition of the presence and/or the absence of the biopolymer.
1. A method for diagnosing insulin resistance by determining the presence of a biopolymer marker consisting of amino acid residues 2–25 of SEQ ID NO:1 comprising:
(a) conducting mass spectrometric analysis on a sample in a manner effective to maximize analysis of peptide fragments contained therein and comparing a mass spectral profile of said biopolymer marker consisting of amino acid residues 2–25 of SEQ ID NO:1 to mass spectral profiles obtained and analyzed from said sample; and
(b) confirming the presence of said biopolymer marker consisting of amino acid residues 2–25 of SEQ ID NO:1 by identifying a mass spectral profile having an ion peak at about 2753 daltons in said sample;
wherein the presence of said biopolymer marker consisting of amino acid residues 2–25 of SEQ ID NO:1 is diagnostic for insulin resistance.
2. The method of claim 1 , wherein said sample is an unfractionated body fluid or a tissue sample.
3. The method of claim 1 , wherein said sample is selected from the group consisting of blood, blood products, urine, saliva, cerebrospinal fluid, and lymph.
4. The method of claim 1 , wherein said mass spectrometric analysis is Surface Enhanced Laser Desorption Ionization (SELDI) mass spectrometry (MS).
5. The method of claim 1 , wherein said sample is from a human.
6. An insulin resistance diagnostic kit comprising: (a) a peptide consisting of amino acid residues 2–25 of SEQ ID NO:1 and (b) an antibody that binds to said peptide.
7. The diagnostic kit of claim 6 , wherein said antibody is immobilized on a solid support.
8. The diagnostic kit of claim 6 , wherein said antibody is labeled.