Transgenic plants incorporating traits of Zostera marina
View Patent ↗The invention provides methods and compositions related to transgenic plants which incorporate genetic traits of the marine eelgrass Zostera marina . These traits include pathogen resistance, which may be conferred by stimulating zosteric acid biosynthesis.
1. An isolated nucleic acid which hybridizes under stringent conditions including a wash step in 0.2×SSC at 65° C. to a nucleic acid consisting of the nucleotide sequence set forth in SEQ ID NO: 15, wherein the isolated nucleic acid encodes a protein having sulfotransferase activity.
2. An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 95% identity with SEQ ID NO: 16, wherein the polypeptide has a sulfotransferase activity.
3. The isolated nucleic acid of claim 2 , encoding a polypeptide comprising an amino acid sequence having at least 99% identity with SEQ ID NO: 16.
4. The isolated nucleic acid of claim 2 , encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 16.
5. The isolated nucleic acid of claim 2 , encoding a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 16.
6. The isolated nucleic acid of claim 1 , wherein the nucleic acid comprises the nucleotide sequence set forth in SEQ ID NO: 15.
7. The isolated nucleic acid of claim 1 , consisting of the nucleotide sequence set forth in SEQ ID NO: 15.
8. A vector comprising the nucleic acid of claim 2 .
9. A vector comprising the nucleic acid of claim 1 .
10. A vector comprising the nucleic acid of claim 3 .
11. An expression vector comprising the nucleic acid of claim 2 .
12. An expression vector comprising the nucleic acid of claim 1 .
13. An expression vector comprising the nucleic acid of claim 3 .
14. A cell comprising the vector of claim 11 .
15. The cell of claim 14 , which is a prokaryotic cell.
16. The cell of claim 14 , which is an eukaryotic cell.
17. A cell comprising the vector of claim 12 .
18. The cell of claim 17 , which is a prokaryotic cell.
19. The cell of claim 17 , which is an eukaryotic cell.
20. A cell comprising the vector of claim 13 .
21. The cell of claim 20 , which is a prokaryotic cell.
22. The cell of claim 20 , which is an eukaryotic cell.
23. A plant comprising a heterologous nucleic acid of claim 2 .
24. The plant of claim 23 , wherein the plant is a monocotyledon.
25. The plant of claim 23 , wherein the plant is a dicotyledon.
26. A plant comprising a heterologous nucleic acid of claim 1 .
27. The plant of claim 26 , wherein the plant is a monocotyledon.
28. The plant of claim 26 , wherein the plant is a dicotyledon.
29. A plant comprising a heterologous nucleic acid of claim 3 .
30. The plant of claim 29 , wherein the plant is a monocotyledon.
31. The plant of claim 29 , wherein the plant is a dicotyledon.