Peptide conjugates and fluorescence detection methods for intracellular caspase assay
View Patent ↗Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.
1. An assay method comprising:
a) delivering a peptide conjugate inside a plurality of eukaryotic cells;
b) illuminating the cells; and
c) detecting a fluorescence signal from at least one of the cells that is indicative of intracellular cleavage of the peptide conjugate,
wherein (i) the conjugate comprises a polypeptide covalently attached to a donor dye through a first linkage and to an acceptor dye through a second linkage, (ii) the donor dye is capable of absorbing light at a first wavelength and emitting excitation energy in response thereto, and the acceptor dye is capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response, and (iii) the donor dye and the acceptor dye each independently comprise a structure selected from Formula I and Formula II below, wherein Formula I comprises the structure:
wherein R 13 , R 14 , R 15 , and R 16 are each independently selected from hydrogen, C 1-C 12 alkyl, C 1-C 12 alkyldiyl, phenyl, substituted phenyl, benzyl, substituted benzyl, biphenyl, substituted biphenyl, naphthyl, substituted naphthyl, heterocycle, substituted heterocycle, a water-solubilizing group and a linkage;
R 13 and R 14 , when taken together, are C 2-C 8 alkyldiyl;
R 15 and R 16 , when taken together, are C 2-C 8 alkyldiyl;
R 13 and R 17 , when taken together, are C 2-C 8 alkyldiyl;
R 15 and R 22 , when taken together, are C 2-C 8 alkyldiyl;
R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , and R 26 are each independetly selected from hydrogen, fluorine, chlorine, bromine, C 1-C 8 alkyl, carboxylate, sulfate, sulfonate, alkylsulfonate, aminomethyl (—CH 2 NH 2 ), aminoalkyl, 4-dialkylaminopyridinium, hydroxymethyl (—CH 2 OH), methoxy (—OCH 3 ), hydroxyalkyl (—ROH), thiomethyl (—CH 2 SH), thioalkyl (—RSH), alkylsulfone (—SO 2 R), arylthio (—SAr), arylsulfone (—SO 2 Ar), sulfonamide (—SO 2 NR 2 ), alkylsulfoxide (—SOR), arylsulfoxide (—SOAr), primary amino (—NH 2 ), ammonium (—NH 3 + ), secondary amino (—NHR), tertiary amino (—NR 2 ), quaternary amine (—NR 3 + ), amido (—CONR 2 ), nitrile (—CN), nitro (—NO 2 ), C 1 -C 8 alkoxy (—OR), phenoxy, phenolic, tolyl, phenyl, C 5 -C 20 aryl, benzyl, heterocycle, phosphonate, phosphate, sulfate, polyethyleneoxy, a water-solubilizing group, and a linkage, wherein R is selected from C 1 -C 6 alkyl and C 1 -C 6 alkyldiyl, and Ar is C 5 -C 14 aryl;
R 19 and R 20 , when taken together, are C 5 -C 14 aryleno;
R 24 and R 25 , when taken together, are C 5 -C 14 aryleno;
with the proviso that at least one of R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , and R 26 is the first linkage or the second linkage;
and Formula II comprises the structure:
wherein R 1 , R 2 , R 3 , and R 4 are each independently selected from hydrogen, C 1 -C 12 alkyl, C 1 -C 12 alkyldiyl, phenyl, substituted phenyl, benzyl, substituted benzyl, biphenyl, substituted biphenyl, naphthyl, substituted naphthyl, heterocycle, substituted heterocycle, a water- solubilizing group, and a linkage;
R 1 and R 2 , when taken together, are C 2 -C 8 alkyldiyl;
R 3 and R 4 , when taken together, are C 2 -C 8 alkyldiyl;
R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently selected from hydrogen, fluorine, chlorine, bromine, C 1 -C 8 alkyl, carboxylate, sulfate, sulfonate, alkylsulfonate, aminomethyl (—CH 2 NH 2 ), aminoalkyl, 4-dialkylaminopyridinium, hydroxymethyl (—CH 2 OH), methoxy (—OCH 3 ), hydroxyalkyl (—ROH), thiomethyl (—CH 2 SH), thioalkyl (—RSH), alkylsulfone (—SO 2 R), arylthio (—SAr), arylsulfone (—SO 2 Ar), sulfonamide (—SO 2 NR 2 ), alkylsulfoxide (—SOR), arylsulfoxide (—SOAr), primary ammo (—NH 2 ), ammonium (—NH 3 + ), secondary amino (—NHR), tertiary amino (—NR 2 ), quaternary amine (—NR 3 + ), amido (—CONR 2 ), nitrile (—CN), nitro (—NO 2 ), C 1 -C 8 alkoxy (—OR), phenoxy, phenolic, tolyl, phenyl, C 5 -C 20 aryl, benzyl, heterocycle, phosphonate, phosphate, sulfate, polyethyleneoxy, a water-solubilizing group, and a linkage, wherein R is selected from C 1 -C 6 alkyl and C 1 -C 6 alkyldiyl, and Ar is C 5 -C 14 aryl;
R 6 and R 7 , when taken together, are C 5 -C 14 aryleno;
R 10 and R 11 , when taken together, are C 5 -C 14 aryleno;
with the proviso that at least one of R 1 , R 2 , R 3 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 is the first linkage or the second linkage.
2. The method of claim 1 wherein the donor dye is capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; and the acceptor dye is capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response.
3. The method of claim 1 further comprising measuring fluorescence intensity after delivering the peptide conjugate inside the cells.
4. The method of claim 1 further comprising measuring fluorescence intensity from the cells before delivering the peptide conjugate inside the cells.
5. The method of claim 1 further comprising counting the cells by detecting fluorescence from the donor dye or the acceptor dye.
6. The method of claim 1 wherein the peptide conjugate is cleaved inside the cell whereby cleavage forms two or more peptide fragments.
7. The method of claim 6 wherein one peptide fragment is comprised of the donor dye and one peptide fragment is comprised of the acceptor dye.
8. The method of claim 6 wherein the peptide conjugate is cleaved by a caspase enzyme.
9. The method of claim 8 wherein the caspase enzyme is caspase-3.
10. The method of claim 1 further comprising the step of delivering an apoptosis inducer inside the cell.
11. The method of claim 1 further comprising the step of delivering a caspase inhibitor inside the cell.
12. The method of claim 1 wherein the fluorescence is detected between 650 to 685 nm.
13. The method of claim 1 wherein the fluorescence is detected between 685 to 720 nm.
14. The method of claim 1 wherein the fluorescence is detected in a first channel between 650 to 685 nm and in a second channel between 685 to 720 nm.
15. The method of claim 14 wherein the fluorescence from the first channel and the fluorescence from the second channel is detected simultaneously.
16. The method of claim 1 wherein the fluorescence is detected by a photomultiplier tube device.
17. The method of claim 1 wherein the fluorescence is detected by a flow cytometer.
18. The method of claim 1 wherein the fluorescence is detected by visualization through a microscope.
19. The method of claim 1 wherein the fluorescence is detected by a CCD device.
20. The method of claim 1 wherein the cells are illuminated by a laser light source.
21. The method of claim 1 wherein the cells are arranged in a plurality of vessels.
22. The method of claim 21 wherein the plurality of vessels are illuminated by a scanning light source.
23. The method of claim 1 wherein the polypeptide comprises the amino acid sequence: Asp-Glu-VAL-Asp (SEQ ID NO:1).
24. The method of claim 1 wherein the polypeptide comprises the amino acid sequence: Gly-Asp-Glu-Val-Asp-Gly-Ile-Lys (SEQ ID NO:2).
25. The method of claim 1 wherein the polypeptide comprises a protected amino acid side-chain.
26. The method of claim 25 wherein the protected amino acid side-chain is a protected glutamic acid side-chain or a protected aspartic acid side-chain.
27. The method of claim 26 wherein the protected glutamic acid side-chain or aspartic acid side-chain is protected as a tert-butyl ester.
28. The method of claim 25 wherein the protected amino acid side-chain is a protected lysine side-chain.
29. The method of claim 25 wherein the protected amino acid side-chain is a protected cysteine side-chain.
30. The method of claim 1 wherein the first linkage is attached to the polypeptide at the side-chain of an amino acid.
31. The method of claim 30 wherein the amino acid is lysine.
32. The method of claim 30 wherein the amino acid is cysteine.
33. The method of claim 1 wherein the first linkage is attached to the polypeptide at its amino terminus.
34. The method of claim 1 wherein the first linkage is attached to the polypeptide at its carboxyl terminus.
35. The method of claim 1 wherein the donor dye comprises Formula I, and the first linkage is attached to the donor dye at R 13 , R 14 , R 15 or R 16 .
36. The method of claim 1 wherein the first linkage comprises C 1 -C 12 alkyldiyl.
37. The method of claim 1 wherein the first linkage comprises an amide bond.
38. The method of claim 1 wherein the first linkage comprises benzyldiyl.
39. The method of claim 1 wherein the second linkage is attached to the polypeptide at the side-chain of an amino acid.
40. The method of claim 39 wherein the amino acid is lysine.
41. The method of claim 1 wherein the second linkage is attached to the polypeptide at its amino terminus.
42. The method of claim 1 wherein the second linkage is attached to the polypeptide at its carboxyl terminus.
43. The method of claim 1 wherein the acceptor dye comprises Formula II, and second linkage is attached to the acceptor dye at R 1 , R 2 , R 3 or R 4 .
44. The method of claim 1 wherein the second linkage comprises C 1 -C 12 alkyldiyl.
45. The method of claim 1 wherein the second linkage comprises an amide bond.
46. The method of claim 1 wherein the second linkage comprises benzyldiyl.
47. The method of claim 1 wherein R 1 and R 2 are each C 1 -C 6 alkyl; R 3 is hydrogen and R 4 is a linkage.
48. The method of claim 1 wherein R 13 and R 14 are each C 1 -C6 alkyl; R 15 is hydrogen and R 16 is a linkage.
49. The method of claim 1 wherein the donor dye comprises Formula I, and Formula I comprises the structure:
50. The method of claim 49 wherein R 18 , R 19 , R 20 , R 21 , R 23 , R 24 , R 25 , and R 26 are hydrogen.
51. The method of claim 1 wherein the donor dye comprises Formula I, and Formula I comprises the structure:
wherein R 15 is a linkage.
52. The method of claim 1 wherein the acceptor dye comprises Formula II, and Formula II comprises the structure:
wherein R 4 is a linkage.
53. The method of claim 1 wherein at least one of R 1 , R 2 , R 3 and R 4 is selected from the structures:
—(CH 2 ) n—NR 2 ,
—(CH 2 ) n— + NR 3 ,
—(CH 2 ) n— + NR 2 —(CH 2 ) n —NR 2 , and
—(CH 2 ) n— + NR 2 —(CH 2 ) n — + NR 3
wherein each n is independently 2 or 3, and each occurrence of R is independently selected from hydrogen, C 1 -C6 alkyl, C 1 -C 6 alkyldiyl, and a linkage.
54. The method of claim 1 wherein at least one of R 1 , R 2 , R 3 , R 4 , R 13 , R 14 , R 15 and R 16 is C 1 -C6 alkylsulfonate or C 4 -C 10 arylsulfonate.
55. The method of claim 54 wherein the C 4 -C 10 arylsulfonate is:
wherein n is 0 or 1.
56. The method of claim 1 wherein at least one of R 1 , R 2 , R 3 , R 4 , R 13 , R 14 , R 15 and R 16 is C 1 -C 6 alkylcarboxylate or C 4 -C 10 arylcarboxylate.
57. The method of claim 56 wherein the C 4 -C 10 arylcarboxylate is:
58. The method of claim 1 wherein the alkyldiyl, substituted phenyl, substituted benzyl, substituted biphenyl, substituted heterocycle and substituted naphthyl is substituted with sulfonate.
59. The method of claim 1 wherein the alkyldiyl, substituted phenyl, substituted benzyl, substituted biphenyl, substituted heterocycle and substituted naphthyl is substituted with carboxyl.
60. The method of claim 1 wherein the water-solubilizing group is carboxylate, sulfonate, phosphonate, phosphate, quaternary amine, sulfate, polyhydroxyl, or water-soluble polymer.
61. The method of claim 1 wherein R 6 and R 7 , when taken together, are [1,2]benzeno, [1,2]naphthaleno or [2,3]naphthaleno.
62. The method of claim 1 wherein R 10 and R 11 , when taken together, are [1,2]benzeno, [1,2]naphthaleno or [2,3]naphthaleno.
63. The method of claim 1 wherein R 19 and R 20 , when taken together, are [1,2]benzeno, [1,2]naphthaleno or [2,3]naphthaleno.
64. The method of claim 1 wherein R 24 and R 25 , when taken together, are [1,2]benzeno, [1,2]naphthaleno or [2,3]naphthaleno.
65. The method of claim 1 wherein the heterocycle is pyrrole, indole, furan, benzofuran, thiophene, benzothiophene, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-quinolyl, 3-quinolyl, 4-quinolyl, 2-imidazole, 4-imidazole, 3-pyrazole, 4-pyrazole, pyridazine, pyrimidine, pyrazine, cinnoline, pthalazine, quinazoline, quinoxaline, 3-(1,2,4-N)-triazolyl, 5-(1,2,4-N)-triazolyl, 5-tetrazolyl, 4-(1-O,3-N)-oxazole, 5-(1-O,3-N)-oxazole, 4-(1-S,3-N)-thiazole, 5-(1-S,3-N)-thiazole, 2-benzoxazole, 2-benzothiazole, 4-(1,2,3-N)-benzotriazole, or benzimidazole.
66. The method of claim 65 , wherein the heterocycle is benzimidazole.
67. The method of claim 1 wherein the peptide is covalently attached by a linkage to a solid support.
68. The method of claim 67 wherein the peptide is covalently attached at the carboxyl terminus to the solid support.
69. The method of claim 67 wherein the solid support is polystyrene.
70. The method of claim 67 wherein the solid support is a polyethyleneoxy/polystyrene graft copolymer.
71. The method of claim 67 wherein the solid support is a bead.
72. The method of claim 67 wherein the solid support is a substantially planar surface.