Method for determining the concentration of thrombin inhibitors and kits therefor
The invention relates to a method for determining the concentration of thrombin inhibitors in a non-turbid body liquid or a non-turbid extract from a body liquid. It comprises the following steps. The body liquid is taken from a living body, and the body liquid is subjected to a separation from the turbid matter, if necessary. To the non-turbid body liquid thus obtained are added a coagulation-inhibiting substance not interfering in the transformation prothrombin/active meizothrombin or Mtdesfgl, resp., a chromogenic or fluorogenic substrate not dissociable by active meizothrombin or Mtdesfgl, resp., and a substance dissociating prothrombin into meizothrombin or Mtdesfgl, resp., and as an optional prothrobmin. The thus obtained solution or mixture, resp., is subjected to a wavelength-selective light absorption or light emission measurement as a function of the time. From the reduction of the light absorption or light emission per time unit is determined the amount of the thrombin inhibitor included in the body liquid by comparison to previously determined standard curves.
1. A single test kit package for determining the concentration of thrombin inhibitors in a non-turbid body liquid or a non-turbid extract from a body liquid, comprising the following components: K1) a solution of a coagulation-inhibiting substance not interfering in the transformants of prothrombin into active meizothrombin or meizothrombin-des fragment 1 , wherein said coagulation-inhibiting substance is a fibrin polymerization inhibiting substance: K2) a chromogenic or fluorogenic substrate dissociable by active meizothrombin or meizothrombin-des fragment 1 ; and K3) a solution of a substance dissociating prothrombin into meizothrombin or meizothrombin-des fragment 1 .
2. A single test kit package for determining the activity of thrombin inhibitors in a non-turbid body or in a non-turbid extract from a body liquid or in a non-turbid non-natural aqueous liquid, comprising the following kit components: K1) a chromogenic or fluorogenic substrate dissociable by active meizothrombin or meizothrombin-des fragment 1 ; K2) meizothrombin or meizothrombin-des fragment 1 ; and K3) a solution of a coagulation-inhibiting substance not interfering in the transformation of prothrombin into active meizothrombin or meizothrombin-des fragment 1 , wherein said coagulation-inhibiting substance is a fibrin polymerization inhibiting substance.
3. A test kit according to claim 1 , further comprising providing a solution of prothrombin.
4. A kit package of claim 1 , further comprising meizothrombin or meizothrombin-des fragment 1 .
5. A single test kit package of claim 1 , wherein the fibrin polymerization inhibiting substance is a Gly-Pro-Arg-Pro tetrapeptide.
6. A single test kit package of claim 2 , wherein the fibrin polymerization inhibiting substance is a Gly-Pro-Arg-Pro tetrapeptide.