Use of antimicrobial peptides as preservatives in ophthalmic preparations, including solutions, emulsions, and suspensions
View Patent ↗Methods for preserving ophthalmic compositions are disclosed. In one embodiment, such compositions include a liquid medium and an antimicrobial component which is preferably substantially non-oxidative. Compositions which include a liquid medium and antimicrobial peptide magainins, present in an amount effective as a preservative, are also disclosed. Preserved compositions useful for administering a therapeutic component to the eyes or caring for contact lenses are also included within the scope of the present invention.
1. An ophthalmic composition comprising a carrier component, and a preservative component selected from the group consisting of magainin antimicrobial peptides, analogs of magainin antimicrobial peptides and mixtures thereof present in an amount effective as a preservative in said composition, wherein said composition is an oil-containing emulsion or an oil-containing suspension.
2. The composition of claim 1 wherein said preservative component is present in an effective amount less than about 10 milligrams per milliliter.
3. The composition of claim 1 which further comprises an effective amount of a buffer component to provide said composition with a desired pH.
4. The composition of claim 1 which further comprises an effective amount of a tonicity component to provide said composition with a desired osmolality.
5. The composition of claim 1 wherein said preservative component is a magainin antimicrobial peptide.
6. The composition of claim 1 wherein said preservative component is an analog of a magainin antimicrobial peptide comprising the amino acid sequence of SEQ ID NO: 4.
7. An ophthalmic composition comprising a carrier component, said composition further comprising an antimicrobial peptide mimetic present in an amount effective as a preservative in said composition, wherein said composition is an oil-containing emulsion or an oil-containing suspension.
8. The composition of claim 7 wherein said antimicrobial peptide mimetic is present in an effective amount less than about 10 milligrams per milliliter.
9. The composition of claim 7 which further comprises water and an effective amount of a buffer component to provide said composition with a desired pH.
10. The composition of claim 7 which further comprises an effective amount of a tonicity component to provide said composition with a desired osmolality.
11. The composition of claim 7 wherein said composition further includes a therapeutically effective component.
12. A method of preserving an ophthalmic composition comprising:
contacting an ophthalmic composition with a magainin analog with an amino acid sequence comprising of SEQ ID NO: 4 in an amount effective as a preservative in said composition, wherein said composition is an oil-containing emulsion or an oil-containing suspension.
13. An ophthalmic composition comprising a magainin antimicrobial analog with an amino acid sequence comprising of SEQ ID NO: 4 present in an amount effective as a preservative in said composition, wherein said composition is provided in a multidose format and wherein said composition is an oil-containing emulsion or an oil-containing suspension.
14. The composition of claim 1 , further comprising an effective amount of a therapeutically effective component.
15. The composition of claim 14 , wherein the therapeutically effective component comprises an antibiotic.
16. The composition of claim 15 , wherein the antibiotic is cyclosporin.
17. The composition of claim 14 , wherein the therapeutically effective component comprises a steroid.
18. The composition of claim 17 , wherein the steroid is prednisolone acetate.
19. The composition of claim 1 , wherein the preservative component is the sole preservative in said composition.
20. The composition of claim 1 , wherein the composition is a surgical irrigant.
21. The composition of claim 1 , further comprising a second antimicrobial component.
22. The composition of claim 21 , wherein the second antimicrobial component is a substantially non-oxidative antimicrobial component.