Anti-CD20 antibodies
View Patent ↗Disclosed are chimeric, immunologically active, isolated, and radiolabeled antibodies directed against the CD20 antigen. The antibodies are useful for treating and diagnosing B cell disorders.
1. An immunologically active chimeric anti-CD20 antibody, wherein the antibody comprises a light chain variable region comprising the amino acid sequence shown as residues 23 to 128 of SEQ ID NO: 4 and a heavy chain variable region comprising the amino acid sequence shown as residues 20 to 140 of SEQ ID NO: 6.
2. The chimeric anti-CD20 antibody of claim 1 which is an IgG1.
3. A composition comprising a chimeric anti-CD20 antibody according to claim 1 and a pharmaceutically acceptable buffer.
4. The composition of claim 3 which is suitable for parenteral administration.
5. The composition of claim 4 wherein the parenteral administration is selected from the group consisting of intravenous, intramuscular, vaginal, intraperitoneal, and subcutaneous administration.
6. The composition of claim 3 which is formulated to deliver an effective dose ranging from about 0.01 to 30 mg/kg body weight upon administration to a patient.
7. The composition of claim 6 wherein the dose ranges from about 0.01 to about 25 mg/kg body weight.
8. The composition of claim 6 wherein the dose ranges from about 0.4 mg to about 20 mg/kg body weight.
9. An isolated anti-CD20 antibody, wherein the antibody comprises a light chain variable region comprising the amino acid sequence shown as residues 23 to 128 of SEQ ID NO: 4 and a heavy chain variable region comprising the amino acid sequence shown as residues 20 to 140 of SEQ ID NO: 6.
10. The anti-CD20 antibody of claim 9 wherein the antibody is murine.
11. A composition comprising an anti-CD20 antibody according to claim 9 and a pharmaceutically acceptable buffer.
12. The chimeric antibody of claim 1 wherein the antibody is not conjugated to a toxin or radioisotope.
13. The chimeric antibody of claim 1 wherein the antibody comprises a human light chain kappa constant region and a human heavy chain gamma 1 constant region.