DNA encoding interleukin-B30
View Patent ↗Purified genes encoding cytokines from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using the reagents and diagnostic kits are also provided.
1. An isolated or recombinant polynucleotide encoding an antigenic polypeptide comprising:
a) at least 17 contiguous amino acids from a mature coding portion of SEQ ID NO: 2,
b) at least 17 contiguous amino acids from a mature coding portion of SEQ ID NO: 4; or
c) at least 17 contiguous amino acids from a mature coding portion of SEQ ID NO: 5.
2. The polynucleotide of claim 1 , encoding a mature polypeptide of:
a) SEQ ID NO: 2;
b) SEQ ID NO: 4; or
c) SEQ ID NO: 5.
3. The polynucleotide of claim 1 , which encodes amino residues 155-164 of SEQ ID NO: 2 and hybridizes under stringent wash conditions of at least 65° C., less than about 150 mM salt to the complement of:
a) the open reading frame of SEQ ID NO: 1; or
b) the open reading frame of SEQ ID NO: 3.
4. The polynucleotide of claim 3 , comprising:
a) at least 67 contiguous nucleotides of a coding portion of SEQ ID NO: 1, wherein said contiguous nucleotides are from nucleotides 466-555 of SEQ ID NO: 1; or
b) at least 67 contiguous nucleotides of a coding portion of SEQ ID NO: 3, wherein said contiguous nucleotides are from nucleotides 580-670 of SEQ ID NO: 3.
5. A recombinant or expression vector comprising said polynucleotide of claim 1 .
6. An isolated host cell comprising said expression vector of claim 5 .
7. A method of making an antigenic polypeptide comprising expressing said recombinant polynucleotide of claim 1 and isolating said polypeptide, thereby making said antigenic polypeptide.
8. Said polynucleotide of claim 1 , wherein said contiguous amino acids number 20.
9. Said polynucleotide of claim 1 , wherein said contiguous amino acids number 30.
10. Said polynucleotide of claim 1 , wherein said contiguous amino acids number 35.
11. Said polynucleotide of claim 1 , wherein said contiguous amino acids number 40.
12. Said polynucleotide of claim 2 , that is a variant due to the degeneracy of the genetic code.
13. The polynucleotide of claim 8 , wherein said wash conditions are
a) at least 70° C.;
b) less than about 100 mM salt; or
c) both a) and b).
14. The polynucleotide of claim 3 , wherein said wash conditions
a) are at least 50% formamide;
b) are less than about 100 mM salt; or
c) are both a) and b).
15. The polynucleotide of claim 1 , that:
a) encodes the mature polypeptide of SEQ ID NO:2,4, or 5; or
b) comprises the mature coding portion of SEQ ID NO: 1 or 3.
16. The polynucleotide of claim 2 , wherein said polynucleotide:
a) encodes a polypeptide with a natural sequence of the mature coding portion of SEQ ID NO: 2 or 4;
b) is isolated from nature;
c) encodes a polypeptide comprising five or fewer conservative substitutions from a natural sequence of SEQ ID NO: 2 or 4;
d) encodes a polypeptide comprising five or fewer conservative substitutions from a natural sequence of SEQ ID NO: 4.
17. The polynucleotide of claim 8 , which:
a) is attached to a solid substrate;
b) is detectably labeled;
c) is in a sterile composition;
d) encodes an antigenic polypeptide having at least 12 amino acid residues; or
e) is synthetically produced.
18. An isolated or recombinant polynucleotide encoding a polypeptide of:
a ) SEQ ID NO: 2 ;
b ) SEQ ID NO: 4 ; or
c ) SEQ ID NO: 5 .
19. A recombinant or expression vector comprising said polynucleotide of claim 18 .
20. An isolated host cell comprising said expression vector of claim 19 .
21. A method of making a polypeptide comprising expressing said recombinant polynucleotide of claim 18 and isolating said polypeptide, thereby making said polypeptide.
22. The polynucleotide of claim 18 , that is a variant due to the degeneracy of the genetic code.
23. An isolated or recombinant polynucleotide that:
a ) encodes the mature polypeptide of SEQ ID NO: 2 , 4 , or 5 ; or
b ) comprises the mature coding portion of SEQ ID NO: 1 or 3 .
24. The polynucleotide of claim 18 , wherein said polynucleotide is isolated from a human or a mouse.
25. The polynucleotide of claim 18 , which:
a ) is attached to a solid substrate;
b ) is detectably labeled;
c ) is in sterile composition; or
d ) is synthetically produced.
26. A recombinant or expression vector comprising said polynucleotide of claim 23 .
27. An isolated host cell comprising said expression vector of claim 26 .
28. A method of making a polypeptide comprising expressing said recombinant polynucleotide of claim 23 and isolating said polypeptide, thereby making said polypeptide.
29. The polynucleotide of claim 23 , that is a variant due to the degeneracy of the genetic code.
30. The polynucleotide of claim 23 , wherein said polynucleotide is isolated from a human or a mouse.
31. The polynucleotide of claim 23 , which:
a ) is attached to a solid substrate;
b ) is detectably labeled;
c ) is in sterile composition; or
d ) is synthetically produced.