Nucleotide sequences which code for the msiK gene
View Patent ↗The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sugar import protein K (msiK) gene, and a host-vector system having a coryneform host bacterium in which the msiK gene is present in enhanced form and a vector which carries at least the msiK gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
1. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1.
2. An isolated polynucleotide comprising the nucleotide sequence of the complete complement of SEQ ID NO: 1.
3. A vector comprising the isolated polynucleotide selected from the group consisting of:
an isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1; and
an isolated polynucleotide comprising the nucleotide sequence of the complete complement of SEQ ID NO: 1.
4. A recombinant host cell of the genus Corynebacterium transformed with the isolated polynucleotide selected from the group consisting of:
an isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1; and
an isolated polynucleotide comprising the nucleotide sequence of the complete complement of SEQ ID NO: 1.
5. The host cell of claim 4 , wherein said host cell is of the species Corynebacterium glutamicum.
6. A recombinant host cell of the genus Corynebacterium or of the species Escherichia coli comprising the vector of claim 3 .
7. The host cell of claim 6 , wherein said host cell is of the species Corynebacterium glutamicum.
8. A method for producing an L-amino acid which comprises
culturing in a medium suitable for producing the L-amino acid a recombinant host cell of the genus Corynebacterium transformed with
an isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1.
9. The method of claim 8 , wherein the host cell is of the species Corynebacterium glutamicum.
10. The method of claim 8 , wherein the L-amino acid is L-Lysine.