Anti-idiotype antibodies to MN proteins and MN polypeptides
View Patent ↗Herein disclosed is a novel oncogene named MN or alternatively MN/CA IX. Abnormal expression of the MN gene is shown to signify oncogenesis, and diagnostic/prognostic methods for pre-neoplastic/neoplastic disease to detect or detect and quantitate such abnormal MN gene expression. Also disclosed are methods to treat pre-neoplastic/neoplastic disease involving the MN gene and protein, e.g., methods comprising the use of MN-specific antibodies, anti-idiotype antibodies thereto, and anti-anti-idiotype antibodies, and the use of MN antisense nucleic acids. Further disclosed are methods to identify and block MN binding site(s) and identify MN protein partners(s).
1. An anti-idiotype antibody to an idiotype of a second antibody, wherein said idiotype of said second antibody specifically binds to an epitope of MN protein, wherein said anti-idiotype antibody comprises an internal image corresponding to said epitope of said MN protein, and wherein said MN protein has an amino acid sepuence of SEQ ID NO: 2 that is encoded by a nucleic acid selected from the group consisting of:
(a) SEQ ID NO: 1; and
(b) polynucleotides that differ from SEQ ID NO: 1 due to the degeneracy of the genetic code.
2. An anti-idiotype antibody according to claim 1 , wherein said second antibody that is specific for said epitope of the MN protein, is either the M75 monoclonal antibody secreted from the hybridoma VU-M75, which was deposited at the American Type Culture Collection under ATCC No. HB 11128, or the MN12 monoclonal antibody that is secreted from the hybridoma MN 12.2.2, which was deposited at the American Type Culture Collection under ATCC No. HB 11647.
3. The anti-idiotype antibody according to claim 1 where said second antibody is an antigen-binding antibody fragment.