Process for purifying antibody
View Patent ↗The present invention relates to a process for purifying an antibody having a desired property, which comprises using a substance having an affinity to a carbohydrate binding to the antibody; a medicament comprising, as an active ingredient, the antibody purified by the process; and a method for diagnosing or preventing various diseases, which comprises using a substance having an affinity to a carbohydrate binding to an antibody.
1. A process for purifying an antibody composition having a desired property, which comprises:
applying a solution containing the antibody composition to a column to which a lectin is immobilized to obtain a non-adsorbed fraction, said lectin being bound to a synthetic resin polymer; and
recovering the antibody composition from the non-adsorbed fraction.
2. The process according to claim 1 , wherein the lectin is at least one lectin selected from the group consisting of a concanavalin A, a wheat germ lectin, a Lens culinaris lectin and a Phaseolus vulgaris lectin E 4 .
3. A process for purifying an antibody composition comprising an antibody having a carbohydrate structure to which bisecting N-acetylglucosamine is bound, which comprises:
applying a solution containing the antibody composition to a column to which a wheat germ lectin or a Phaseolus vulgaris lectin E 4 is immobilized to adsorb the antibody composition to the column, said lectin being bound to a synthetic resin polymer;
eluting the antibody composition from the column with an eluent to obtain an eluted fraction; and
recovering the antibody composition from the eluted fraction.
4. A process for purifying an antibody composition having a higher antibody-dependent cell-mediated cytotoxic activity than the antibody composition before purification, which comprises:
applying a solution containing the antibody composition to a column to which a wheat germ lectin or a Phaseolus vulgaris lectin E 4 is immobilized to adsorb the antibody composition to the column;
eluting the antibody composition from the column with an eluent to obtain an eluted fraction; and
recovering the antibody composition from the eluted fraction.
5. The process according to claim 4 , wherein the lectin is immobilized to a synthetic resin polymer.
6. A process for purifying an antibody composition comprising an antibody having a carbohydrate structure to which fucose is not bound, which comprises:
applying a solution containing the antibody composition to a column to which a Lens culinaris lectin is immobilized to obtain a non-adsorbed fraction, said lectin being bound to a synthetic resin polymer; and
recovering the antibody composition from the non-adsorbed fraction.
7. A process for purifying an antibody composition having a higher antibody-dependent cell-mediated cytotoxic activity than the antibody composition before purification, which comprises:
applying a solution containing the antibody composition to a column to which a Lens culinaris lectin is immobilized to obtain a non-adsorbed fraction; and
recovering the antibody composition from the non-adsorbed fraction.
8. The process according to claim 7 , wherein the lectin is immobilized to a synthetic resin polymer.