Cold storage solution for organ and biological tissue preservation
View Patent ↗Cold storage solutions for the preservation of organs and biological tissues prior to implantation, including a prostaglandin having vasodilatory, membrane stabilizing, platelet aggregation prevention upon reperfusion, and complement activation inhibitory properties, a nitric oxide donor, and a glutathione-forming agent.
1. An organ or biological tissue preservation aqueous cold storage solution comprising:
about 1,000 micrograms/L prostaglandin E1,
about 10 mg/L nitroglycerin,
about 0.2 mg/L N-acetylcysteine,
about 50–150 mM potassium lactobionate,
about 10–40 mM KH 2 PO 4 ,
about 2–8 mM MgSO 4 ,
about 10–50 mM raffinose,
about 1–20 mM adenosine,
about 1–10 mM allopurinol, and
about 40–60 g/L pentastarch.
2. An organ or biological tissue preservation aqueous cold storage solution comprising:
about 1,000 micrograms/L prostaglandin E1,
about 10 mg/L nitroglycerin,
about 0.2 mg/L N-acetylcysteine,
about 75–125 mM potassium lactobionate,
about 20–30 mM KH 2 PO 4 ,
about 3–7 mM MgSO 4 ,
about 20–40 mM raffinose,
about 2–10 mM adenosine,
about 1–5 mM allopurinol, and
about 45–55 g/L pentastarch.
3. An organ or biological tissue preservation aqueous cold storage solution comprising:
about 1,000 micrograms/L prostaglandin E1,
about 10 mg/L nitroglycerin,
about 0.2 mg/L N-acetylcysteine,
about 100 mM potassium lactobionate,
about 25 mM KH 2 PO 4 ,
about 5 mM MgSO 4 ,
about 30 mM raffinose,
about 5 mM adenosine,
about 1 mM allopurinol, and
about 50 g/L pentastarch.
4. The cold storage solution of claims 1 , 2 , or 3 , further comprising NaCl and KOH.
5. The cold storage solution of claim 1 , further comprising sterile water.
6. The cold storage solution of claim 5 , further comprising about 700–900 mls sterile water.
7. A method for preserving an organ or biological tissue comprising:
flushing at least one of a cadaveric organ and tissue with the cold storage solution of claim 1 , 2 or 3 ;
allowing the flushed at least one of a cadaveric organ and tissue to be enveloped in the cold storage solution; and
storing the at least one of a cadaveric organ and tissue in the cold storage solution in at least one of a deep hypothermic condition and physiological condition.
8. The method of claim 7 wherein the flushing comprises:
infusing the solution into vasculature of the at least one of a cadaveric organ and tissue; and exsanguinating the at least one of a cadaveric organ and tissue.
9. The method of claim 7 wherein the storing comprises:
replacing blood in vasculature of the at least one of a cadaveric organ and tissue with the solution.
10. The method of claim 7 further comprising:
replacing the solution with at least blood to return the at least one of a cadaveric organ and tissue to a normothermic condition.
11. The method of claim 7 further comprising:
cooling the solution to below 10° C.;
inspecting the cooled solution for precipitates; and
removing any precipitates by filtration.
12. A method of preparing the organ or biological tissue preservation solution of claim 1 , 2 or 3 comprising:
providing a solution with sterile water;
adding the potassium lactobionate, the potassium phosphate, the raffinose, the adenosine, the allopurinol and the pentastarch to the solution; and
mixing the prostaglandin E1, the nitroglycerin and the N-acetylcysteine into the solution.
13. The method of claim 12 further comprising:
mixing the solution until all components are dissolved.
14. The method of claim 12 further comprising:
infusing the pentastarch under pressure through a dialyzing filter;
centrifuging the prostaglandin E1 under hypothermic conditions; and
filtering the centrifuged prostaglandin E1.