Cellulose synthase encoding polynucleotides and uses thereof
View Patent ↗The invention relates to isolated polynucleotides encoding functional cellulose synthases and UDP-glucose binding domain thereof, transgenic plants and plant cells transformed with the polynucleotides. The invention further relates to methods of transforming plants and plant cells with cellulose synthase or UDP-encoding polynucleotides.
1. An isolated polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has cellulose synthase activity.
2. The polynucleotide of claim 1 , wherein the polypeptide comprises SEQ ID NO: 2.
3. The polynucleotide of claim 1 , wherein the polynucleotide comprises the nucleotide sequence from position 660 to position 2250 of SEQ ID NO: 1.
4. A transgenic plant cell comprising an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has cellulose synthase activity.
5. A transgenic plant comprising the plant cell of claim 4 .
6. The transgenic plant of claim 5 , wherein the plant is a tree.
7. A vector comprising an isolated polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has cellulose synthase activity and wherein the polynucleotide is operably linked with a promoter sequence functional in a plant.
8. A transgenic plant cell comprising the vector of claim 7 .
9. A method for producing a transgenic plant cell comprising introducing into the plant cell an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has cellulose synthase activity.
10. The method of claim 9 , wherein the polynucleotide is operably associated with a promoter.
11. The method of claim 9 , wherein the polynucleotide comprises nucleotides from position 660 to position 2250 of SEQ ID NO: 1.
12. The method of claim 9 , wherein the polynucleotide comprises the nucleotide sequence from position 69 to position 3005 of SEQ ID NO: 1.
13. The method of claim 12 , wherein the polynucleotide is expressed in the anti-sense orientation.
14. A method of producing a transgenic plant cell comprising introducing into the plant cell a vector comprising a promoter operably linked to a polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has cellulose synthase activity.
15. The method of claim 14 , wherein the polypeptide comprises SEQ ID NO: 2.
16. The method of claim 14 , wherein the polynucleotide comprises the nucleotide sequence from position 660 to position 2250 of SEQ ID NO: 1.
17. The method of claim 15 , wherein the polynucleotide comprises the nucleotide sequence from position 69 to position 3005 of SEQ ID NO: 1.
18. The method of claim 14 , wherein the polynucleotide comprises SEQ ID NO: 1.
19. The method of claim 17 , wherein the polynucleotide is expressed in the anti-sense orientation.
20. The method of claim 16 , wherein the polynucleotide is expressed in the anti-sense orientation.
21. A method for producing a transgenic plant comprising introducing into the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has cellulose synthase activity.
22. A transgenic plant comprising the vector of claim 7 .
23. The transgenic plant of claim 22 , which is a tree.
24. An isolated polynucleotide encoding a polypeptide comprising the amino acid sequence from position 220 to position 749 of SEQ ID NO: 2, wherein the polypeptide has UDP-glucose binding activity.
25. The polynucleotide of claim 24 , wherein the polypeptide comprises SEQ ID NO: 2.
26. The polynucleotide of claim 24 , wherein the polynucleotide comprises the nucleotide sequence from position 660 to position 2250 of SEQ ID NO: 1.