Marine actinomycete taxon for drug and fermentation product discovery
View Patent ↗The invention is the discovery of an actinomycete genus, given the name Salinospora gen. nov., that displays an obligate requirement of seawater (Na + ) for growth and unique 16S rRNA signature nucleotides. The invention is also the use of the genus for the production and discovery of active biomolecules such as pharmaceutical agents, agrichemicals, immunomodifiers, enzymes and enzyme inhibitors.
1. An isolated marine actinomycete having an obligate requirement of sodium for growth, wherein the marine actinomycete is a strain of Salinospora comprising 16S rRNAs SEQ ID NO:4 or 5.
2. The strain of Salinospora of claim 1 , wherein the strain of Salinospora is obtained from sediment.
3. The isolated strain of Salinospora of claim 1 , wherein SEQ ID NOs:4 or 5 have signature nucleotides not found in other Micromonosporaceae 16S rRNAs.
4. The isolated strain of Salinospora of claim 3 , wherein the signature nucleotides are numbered and identified in accordance to the E.coli 16S rRNA sequence, wherein a uridine or cytosine at position 207 is an adenosine; an adenosine or guanine at position 366 is a cytosine; an adenosine or guanine at position 467 is a uracil; an adenosine at position 468 is a uracil; and adenosine at position 1456 is a guanine.
5. The method for producing a biomolecule, comprising
culturing a strain of Salinospora of claim 1 in a sodium containing growth media, wherein the biomolecule is produced by the strain of Salinospora ;
collecting the strain of Salinospora or the sodium containing growth media containing the biomolecule; and extracting the biomolecule from the strain of Salinospora or the sodium containing growth media, thereby producing the biomolecule.
6. The method of claim 5 , wherein the growth media comprises sodium at a concentration of 450 mM.
7. An isolated strain of Salinospora having an obligate requirement of sodium for growth, wherein the strain of Salinospora is a strain of Salinospora comprising 16S rRNAs SEQ ID NO:4 or 5 and signature nucleotides therein, wherein the signature nucleotides are numbered and identified in accordance to the E.coli 16S rRNA sequence, wherein a uridine or cytosine at position 207 is an adenosine; an adenosine or guanine at position 366 is a cytosine; an adenosine or guanine at position 467 is a uracil; an adenosine at position 468 is a uracil; and adenosine at position 1456 is a guanine.
8. An isolated strain of Salinospora having an obligate requirement of sodium for growth, wherein the strain of Salinospora is a strain of Salinospora comprising a 16S rRNA and signature nucleotides therein, wherein the signature nucleotides are numbered and identified in accordance to the E.coli 16S rRNA sequence, wherein a uridine or cytosine at position 207 is an adenosine; an adenosine or guanine at position 366 is a cytosine; an adenosine or guanine at position 467 is a uracil; an adenosine at position 468 is a uracil; and adenosine at position 1456 is a guanine.
9. The strain of Salinospora of claims 4 , 7 or 8 , wherein the strain of Salinospora is obtained from sediment.