Betaine/GABA transport protein biopolymer marker indicative of insulin resistance
View Patent ↗The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of at least one disease state relative to recognition of the presence and/or the absence of the biopolymer, predict disease risk assessment, and develop therapeutic avenues against the disease.
1. An isolated biopolymer marker peptide consisting of SEQ ID NO: 2 diagnostic for insulin resistance.
2. A method for diagnosing insulin resistance comprising:
(a) obtaining a sample from a patient;
(b) conducting mass spectrometric analysis on said sample to elucidate peptide fragments contained therein; and
(c) comparing mass spectrum profiles of an isolated biopolymer marker peptide consisting of SEQ ID NO:2 to mass spectrum profiles of peptides elucidated from said sample; wherein recognition of a mass spectrum profile in the sample displaying the characteristic profile of the mass spectrum profile for the isolated biopolymer marker peptide consisting of SEQ ID NO:2 having a molecular weight of about 1212 daltons is diagnostic for insulin resistance.
3. The method of claim 2 , wherein said sample as an unfractionated body fluid or a tissue sample.
4. The method of claim 2 , wherein said sample is selected from the group consisting of blood, blood products, urine, saliva, cerebrospinal fluid, and lymph.
5. The method of claim 2 , wherein said mass spectrometric analysis is selected from the group consisting of Surface Enhanced Laser Desorption Ionization (SEIJDI) mass spectrometry (MS), Matrix-Assisted Laser Desorption/ Quadrupole quadrupole Time-Of-Flight (MALDI-TOF) mass spectrometry, multiple sequential Mass Spectrometry (MS/MS), multiple sequential Time-Of-Flight/Time-of-Flight (TOF-TOF) mass spectrometry, Electrospray Ionization/Ionization Time-Of-Flight (ESI-O-TOF) mass spectrometry and lON-TRAP mass spectrometry.
6. The method of claim 2 , wherein said patient is a human.
7. An insulin resistance diagnostic kit comprising: (a) a biopolymer marker peptide consisting of SEQ ID NO: 2 and (b) an antibody that binds to said biopolymer marker pepbide in a sample from a patient.
8. The insulin resistance diagnostic kit of claim 7 , wherein said antibody is immobilized on a solid support.
9. The insulin resistance diagnostic kit of claim 7 , wherein said antibody is labeled.