Plasmin variants and uses thereof
View Patent ↗The invention concerns plasmin variants. Polynucleotides the disclosed plasmin variants are provided. Additionally, methods of using the plasmin polynucleotides and polynucleotides are provided herein.
1. A Plasminute polypeptide encoded by an isolated polynucleotide consisting of an open reading frame of the human cDNA of deposited clone 789749 — 182-14-3-0-C12-F.
2. An isolated Plasminute protease consisting of amino acids 1 to 207 of SEQ ID NO:54.
3. The protease of claim 2 , wherein said protease is a serine protease.
4. A composition comprising the protease of claim 2 and a physiologically acceptable carrier.
5. A composition comprising the polypeptide of claim 3 and a physiologically acceptable carrier.
6. A composition comprising the polypeptide of claim 1 and a physiologically acceptable carrier.
7. A method of digesting a protein consisting of contacting a protein with a) a Plasminute polypeptide encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 789749 — 182-14-3-0-C12-F; b) a Plasminute protease consisting of amino acids 1 to 207 of SEQ ID NO:54; or c) compositions thereof under conditions that allow for the digestion of said protein.
8. The method of claim 7 , wherein a protein is contacted with a Plasminute polypeptide encoded by an isolated polynucleotide consisting of an open reading frame of the human cDNA of deposited clone 789749 — 182-14-3-0-C12-F.
9. The method of claim 7 , wherein a protein is contacted with a Plasminute protease consisting of amino acids 1 to 207 of SEQ ID NO:54.
10. The method of claim 7 , wherein a protein is contacted with a composition comprising a carrier and a Plasminute protease consisting of amino acids 1 to 207 of SEQ ID NO:54.
11. The method of claim 7 , wherein a protein is contacted with a composition consisting of a carrier and a Plasminute polypeptide encoded by an isolated polynucleotide consisting of an open reading frame of the human cDNA of deposited clone 789749 — 182-14-3-0-C12-F.