Serine carboxypeptidase hx (SCPhx) and compositions thereof
View Patent ↗The invention concerns GENSET polynucleotides and polypeptides. Such GENSET products may be used as reagents in forensic analyses, as chromosome markers, as tissue/cell/organelle-specific markers, in the production of expression vectors. In addition, they may be used in screening and diagnosis assays for abnormal GENSET expression and/or biological activity and for screening compounds that may be used in the treatment of GENSET-related disorders.
1. An isolated or purified serine carboxypeptidase (SCPhx) polypeptide:
a) encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-A11-F (ATCC PTA-2732);
b) comprising amino acids −26 to 267 of SEQ ID NO: 4;
c) comprising amino acids 1 to 267 of SEQ ID NO: 4;
d) consisting of amino acids −26 to 267 of SEQ ID NO: 4; or
e) consisting of amino acids 1 to 267 of SEQ ID NO:4.
2. The isolated or purified SCPhx polypeptide of claim 1 , wherein said polypeptide is encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-A11-F (ATCC PTA-2732).
3. The isolated or purified SCPhx polypeptide of claim 1 , wherein said polypeptide comprises amino acids −26 to 267 of SEQ ID NO: 4.
4. The isolated or purified SCPhx polypeptide of claim 1 , wherein said polypeptide comprises amino acids 1 to 267 of SEQ ID NO: 4.
5. The isolated or purified SCPhx polypeptide of claim 1 , wherein said polypeptide consists of amino acids −26 to 267 of SEQ ID NO: 4.
6. The isolated or purified SCPhx polypeptide of claim 1 , wherein said polypeptide consists of amino acids 1 to 267 of SEQ ID NO:4.
7. A composition comprising a physiologically acceptable carrier and a serine carboxypeptidase (SCPhx) polypeptide:
a) encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-11-F (ATCC PTA-2732);
b) comprising amino acids −26 to 267 of SEQ ID NO: 4;
c) comprising amino acids 1 to 267 of SEQ ID NO: 4;
d) consisting of amino acids −26 to 267 of SEQ ID NO: 4; or
e) consisting of amino acids 1 to 267 of SEQ ID NO:4.
8. The composition of claim 7 , wherein said SCPhx polypeptide is encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-A11-F (ATCC PTA-2732).
9. The composition of claim 7 , wherein said SCPhx polypeptide comprises amino acids −26 to 267 of SEQ ID NO: 4.
10. The composition of claim 7 , wherein said SCPhx polypeptide comprises amino acids 1 to 267 of SEQ ID NO: 4.
11. The composition of claim 7 , wherein said SCPhx polypeptide consists of amino acids −26 to 267 of SEQ ID NO: 4.
12. The composition of claim 7 , wherein said SCPhx polypeptide consists of amino acids 1 to 267 of SEQ ID NO:4.
13. A method of making a serine carboxypeptidase (SCPhx) polypeptide, said method comprising:
a) providing a population of cells comprising a polynucleotide encoding a SCPhx polypeptide operably linked to a promoter, said SCPhx polypeptide:
i) being encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-A11-F (ATCC PTA-2732);
ii) comprising amino acids −26 to 267 of SEQ ID NO: 4;
iii) comprising amino acids 1 to 267 of SEQ ID NO: 4;
iv) consisting of amino acids −26 to 267 of SEQ ID NO: 4; or
v) consisting of amino acids 1 to 267 of SEQ ID NO:4;
b) culturing said population of cells under conditions conducive to the production of said polypeptide within said cells; and
c) purifying said polypeptide from said population of cells.
14. The method of claim 13 , wherein said polynucleotide is encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-A11-F (ATCC PTA-2732).
15. The method of claim 13 , wherein said polynucleotide encodes a polypeptide comprising amino acids −26 to 267 of SEQ ID NO: 4.
16. The method of claim 13 , wherein said polynucleotide encodes a polypeptide comprising amino acids 1 to 267 of SEQ ID NO: 4.
17. The method of claim 13 , wherein said polynucleotide encodes a polypeptide consisting of amino acids −26 to 267 of SEQ ID NO: 4.
18. A method of binding a SCPhx polypeptide according to claim 1 to an antibody comprising contacting said antibody with said polypeptide under conditions in which said antibody can specifically bind to said polypeptide.
19. The method of claim 18 , wherein said SCPhx polypeptide is encoded by an isolated polynucleotide comprising an open reading frame of the human cDNA of deposited clone 1000848582 — 181-40-4-0-A11-F (ATCC PTA-2732).
20. The method of claim 18 , wherein said SCPhx polypeptide comprises amino acids −26 to 267 of SEQ ID NO: 4.
21. The method of claim 18 , wherein said SCPhx polypeptide comprises amino acids 1 to 267 of SEQ ID NO: 4.
22. The method of claim 18 , wherein said SCPhx polypeptide consists of amino acids −26 to 267 of SEQ ID NO: 4.
23. The method of claim 18 , wherein said SCPhx polypeptide consists of amino acids 1 to 267 of SEQ ID NO:4.