Glycoprotein and apolipoprotein biopolymer markers predictive of Alzheimers disease
The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of said at least one disease state relative to recognition of the presence and/or the absence of said biopolymer, predict disease risk assessment, and develop therapeutic avenues against said disease.
1 . An isolated biopolymer marker consisting of amino acid residues 2-18 of SEQ ID NO:1 which evidences a link to Alzheimer's disease.
2 - 38 . (canceled)
39 . A method for determining the presence of a biopolymer marker consisting of amino acid residues 2-18 of SEQ ID NO:1 comprising:
(a) obtaining a sample from a patient;
(b) conducting mass spectrometric analysis on said sample in a manner effective to maximize analysis of peptide fragments contained therein and comparing a mass spectral profile of said biopolymer marker consisting of amino acid residues 2-18 of SEQ ID NO:1 to mass spectral profiles of peptides obtained and analyzed from said sample; and
(c) confirming the presence of said biopolymer marker consisting of amino acid residues 2-18 of SEQ ID NO:1 in said sample by identifying a mass spectral profile having an ion peak at about 1874 daltons;
wherein the presence of said biopolymer marker consisting of amino acid residues 2-18 of SEQ ID NO:1 is indicative of a link to Alzheimer's disease.
40 . The method of claim 39 , wherein said sample is an unfractionated body fluid or a tissue sample.
41 . The method of claim 39 , wherein said sample is selected from the group consisting of blood, blood products, urine, saliva, cerebrospinal fluid, and lymph.
42 . The method of claim 39 , wherein said mass spectrometric analysis is selected from the group of mass spectrometry formats consisting of Surface Enhanced Laser Desorption Ionization (SELDI) mass spectrometry (MS), Maldi Qq TOF, MS/MS, TOF-TOF, ESI-Q-TOF and ION-TRAP.
43 . The method of claim 39 , wherein said patient is a human.
44 . A kit for determining the presence of a biopolymer marker consisting of amino acid residues 2-18 of SEQ ID NO:1 comprising: (a) a peptide consisting of amino acid residues 2-18 of SEQ ID NO:1, and (b) an antibody that binds to said peptide in a sample from a patient.
45 . The kit of claim 44 , wherein said antibody is immobilized on a solid support.
46 . The kit of claim 44 , wherein said antibody is labeled.