Optical biodiscs with reflective layers
View Patent ↗An optical biodisc has a substrate, a first reflective layer over the substrate, an opening in the first reflective layer for receiving an investigational feature, and a second reflective layer over the opening for reflecting light transmitted through the substrate.
1. A method for forming an optical disc, comprising:
forming a first reflective layer over a substrate such that there is at least a first portion without the first reflective layer;
forming a second reflective layer on a second portion of a cap; and
assembling the substrate and cap so that the substrate and cap are parallel along a first direction and the first portion and second portion are aligned along a second direction perpendicular to the first direction such that the second portion of the cap covers the first portion without the first reflective layer.
2. The method of claim 1 , wherein the first reflective layer is formed over the substrate and a portion is removed.
3. The method of claim 1 , wherein the first reflective layer is patterned over the substrate so that it is provided on the substrate with the first portion lacking.
4. The method of claim 1 , further comprising forming a capture layer over the first portion, the capture layer adapted to receive a biological material for capturing a reporter introduced into a volume between the first and second portions.
5. The method of claim 1 , further comprising providing a channel layer between the substrate and cap, the channel layer having a pattern cut out, such that when the substrate, cap, and channel layer are assembled, the pattern in the channel layer defines a fluidic channel in the optical disc.
6. The method of claim 5 , further comprising forming an opening in the cap over a portion of the fluidic channel.