Transgenic transchromosomal rodents for making human antibodies
View Patent ↗The present invention provides novel transgenic nonhuman mammals capable of producing human sequence antibodies, as well as methods of producing and using these antibodies.
1. A transgenic mouse comprising two human immunoglobulin loci, wherein one of said human immunoglobulin loci is a human heavy chain locus and the other locus is a human light chain locus; wherein the human heavy chain locus is carried by transchromosome SC20 and the human light chain locus is carried by a transgene integrated into the genome of said mouse; and wherein an endogenous mouse heavy chain locus and at least one endogenous mouse light chain locus are inactivated.
2. The transgenic mouse of claim 1 , wherein the endogenous mouse heavy chain locus and the endogenous mouse kappa light chain locus are inactivated.
3. The transgenic mouse of claim 1 , wherein at least a part of the human light chain locus is cloned into a YAC vector.
4. The transgenic mouse of claim 1 , wherein the transgene is the KCo5 transgene.