Goodpasture's syndrome model mouse
View Patent ↗The present invention provides a non-human model animal of Goodpasture's syndrome that contributes to the treatment of Goodpasture's syndrome where the development of therapy had been delayed due to the lack of adequate disease models, a method for screening a remedy for Goodpasture's syndrome by using the model animal, and a method for diagnosing Goodpasture's syndrome at the early stage. A Goodpasture's syndrome model mouse is constructed by immunizing immunoglobulin Fcγ receptor IIB knockout mouse with type IV collagen, thereby inducing Goodpasture's syndrome. Moreover, a remedy for Goodpasture's syndrome is screened by administration of test substances to the Goodpasture's syndrome model mouse, followed by evaluating the severity of the expression of Goodpasture's syndrome as an index, such as diffuse alveolar hemorrhage, glomerulonephritis, the appearance of antikidney glomerular basement membrane antibody, and the like.
1. A model mouse showing symptoms of diffuse alveolar hemorrhage, glomerulonephritis, and the appearance of antikidney glomerular basement membrane antibody, wherein the model mouse is obtained by immunizing with type IV collagen a homozygous transgenic mouse whose genome comprises a disruption of the FcγRIIB gene wherein said transgenic mouse does not produce FcγRIIB protein.
2. A method for screening a test substance for improving symptoms of diffuse alveolar hemorrhage, glomerulonephritis, and the appearance of antikidney glomerular basement membrane antibody, comprising the steps of:
a) administering a test substance to a first model mouse showing the symptoms of diffuse alveolar hemorrhage, glomerulonephritis, and the appearance of antikidney glomerular basement membrane antibody wherein the model mouse is obtained by immunizing with type IV collagen a homozygous transgenic mouse whose genome comprises a disruption of the FcγRIIB gene wherein said transgenic mouse does not produce FcγRIIB protein,
b) determining at least one symptom selected from the group consisting of diffuse alveolar hemorrhage, glomerulonephritis, and the appearance of antikidney glomerular basement membrane antibody,
c) performing a comparative evaluation with a second model mouse used as control to which the test substance is not administered, wherein a decrease in the severity of at least one said symptom in the first model mouse to which the test substance is administered compared to the second model mouse to which the test substance is not administered indicates that the test substance is effective to improve said symptom.