PRO274 nucleic acids
View Patent ↗The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
1. An isolated nucleic acid comprising:
(a) the extracellular domain coding sequence from within the nucleic acid sequence of SEQ ID NO:6;
(b) the nucleic acid sequence of SEQ ID NO:6;
(c) the full-length coding sequence from within the nucleic acid sequence of SEQ ID NO:6; or
(d) the full-length coding sequence of the cDNA deposited under ATCC accession number 209786.
2. The isolated nucleic acid of claim 1 comprising the extracellular domain coding sequence from within the nucleic acid sequence of SEQ ID NO:6.
3. The isolated nucleic acid of claim 1 comprising the nucleic acid sequence of SEQ ID NO:6.
4. The isolated nucleic acid of claim 1 comprising the full-length coding sequence of the nucleic acid sequence of SEQ ID NO:6.
5. The isolated nucleic acid of claim 1 comprising the full-length coding sequence of the cDNA deposited under ATCC accession number 209786.
6. A vector comprising the nucleic acid of claim 1 .
7. The vector of claim 6 , wherein said nucleic acid is operably linked to control sequences recognized by a host cell transformed with the vector.
8. A host cell comprising the vector of claim 6 .
9. The host cell of claim 8 , wherein said cell is a CHO cell, an E. coli or a yeast cell.
10. An isolated nucleic acid molecule consisting of an at least 20 nucleotide fragment of the nucleic acid sequence of SEQ ID NO:6, or a complement of said fragment, that specifically hybridizes under stringent conditions to:
(a) the nucleic acid sequence of SEQ ID NO: 6 or a complement thereof; or
(b) the full-length coding sequence of the cDNA deposited under ATCC accession number 209786 or a complement thereof;
wherein, said stringent conditions use 50% formamide, 5×SSC, 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 50% formamide at 55° C., followed by a wash comprising of 0.1×SSC containing EDTA at 55° C.
11. The isolated nucleic acid molecule of claim 10 that is at least 50 nucleotides.
12. The isolated nucleic acid molecule of claim 10 that is at least 60 nucleotides.
13. The isolated nucleic acid molecule of claim 10 that is at least 70 nucleotides.
14. The isolated nucleic acid molecule of claim 10 that is at least 80 nucleotides.
15. The isolated nucleic acid molecule of claim 10 that is at least 90 nucleotides.
16. The isolated nucleic acid molecule of claim 10 that is at least 100 nucleotides.