Amylase variants
View Patent ↗The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca 2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.
1. A variant of a parent Bacillus stearothermophilus alpha-amylase, wherein the variant has an amino acid sequence which has at least 95% homology to the parent Bacillus stearothermophilus alpha-amylase and comprises a deletion of amino acids 179 an 180, using SEQ ID NO:3 for numbering, and wherein the variant has alpha-amylase activity.
2. The variant of claim 1 , wherein the variant further comprises a substitution of a cysteine at amino acids 349 and 428, using SEQ ID NO:3 for numbering.
3. A variant alpha-amylase, wherein the variant has at least 95% homology to SEQ ID NO:3 and comprises a deletion of amino acids 179 and 180, using SEQ ID NO:3 for numbering and wherein the variant has alpha-amylase activity.
4. The variant of claim 3 , wherein the variant further comprises a substitution of a cysteine at amino acids 349 and 428, using SEQ ID NO:3 for numbering.
5. A variant of a Bacillus stearothermophilus alpha-amylase, wherein the alpha-amylase variant consists of a deletion of amino acids 179 and 180, using SEQ ID NO:3 for numbering.