Hematopoietic growth factor inducible neurokinin-1 gene
View Patent ↗Bone marrow (BM) is the major organ where immune cells are derived. Homeostasis in the BM is maintained by inter- and intra-cellular interactions by the various subsets of BM cells. The present invention discloses the cloning of a new cDNA from stimulated BM stromal cells that was retrieved with a probe specific for the neurokinin-1 (NK-1) receptor. The cloned cDNA was designated ‘Hematopoietic Growth Factor Inducible Neurokinin-1 type’ (HGFIN) gene based on its expression in differentiated hematopoietic cells. Hence, the present invention provides a novel gene, HGFIN, which encodes a protein receptor that is involved in the regulation of hematopoietic proliferation and differentiation. The protein of the present invention may be involved as a central mediator of white blood cell, progenitor, differentiation, and therefore, may be useful in the prevention and treatment of lymphoproliferative syndromes such as B-cell related maladies, including but not limited to acute and chronic myeloid and lymphocytic leukemia as well as the B-cell subtype of Hodgkin's and non-Hodgkin's lymphomas.
1. An isolated polynucleotide, comprising a Hematopoietic Growth Factor Inducible Neurokinin-I type nucleotide sequence that has at least 97% identity to SEQ ID NO:1, said identity being calculated over the entire length of SEQ ID NO:1.
2. The polynucleotide of claim 1 , comprising the nucleotide sequence of SEQ ID NO:1.
3. A vector comprising a polynucleotide of claim 1 , wherein said polynucleotide encodes a Hematopoietic Growth Factor Inducible Neurokinin-I type polypeptide.
4. The vector of claim 3 , wherein the Hematopoietic Growth Factor Inducible Neurokinin-I type polypeptide further comprises the amino acid sequence of SEQ ID NO:2.
5. An isolated host cell comprising the vector of claim 3 .
6. A process for producing an Hematopojetic Growth Factor Inducible Neurokinin-I type polypeptide comprising culturing a host of claim 5 under conditions sufficient for the production of said polypeptide and recovering the polypeptide from the culture.
7. A process for producing a cell which produces a Hematopoietic Growth Factor Inducible Neurokinin-I type polypeptide comprising transforming, transducing or transfecting an isolated host cell with the vector of claim 3 such that the host cell, under appropriate culture conditions, produces an HGFIN polypeptide.
8. The process of claim 7 , wherein the cell is a bone marrow derived cell removed from the body of a subject.