Method for reductive amination of a ketone using a mutated enzyme
Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amino acid from a target 2-ketoacid, the production of an amine from a target ketone and the production of an alcohol from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid or ketone or the reduction of the ketone and providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid or ketone under conditions sufficient to permit the formation of the desired amino acid, amine or alcohol to thereby produce the amino acid, amine or alcohol.
1. A method for producing an amine from a target ketone, comprising:
creating a mutated enzyme that catalyzes reductive amination of the target ketone; and
providing the mutated enzyme in a reaction mixture comprising the target ketone under conditions sufficient to permit the formation of the corresponding amine to thereby produce the amine,
wherein the ketone is not a 2-ketoacid.
2. The method of claim 1 , wherein the mutated enzyme is an amino acid dehydrogenase.
3. The method of claim 2 , further comprising providing an existing amino acid dehydrogenase, wherein the mutated enzyme is created by mutating the existing amino acid dehydrogenase, and further wherein the mutated enzyme catalyzes reductive amination of the target ketone at a greater rate than the existing amino acid dehydrogenase.