IP Library › Granted Patent US 6,921,656
Granted Patent B1
US 6,921,656 · App. 10/048,621 · Granted Jul 26, 2005

Polypeptides having glucoamylase activity

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Quick Facts
Patent No.
US 6,921,656
App. No.
10/048,621
Granted
Jul 26, 2005
Kind
B1
Abstract

Polypeptides having the amino acid sequence represented by SEQ ID NO:6 in Sequence Listing or an amino acid sequence derived from the above sequence by at least one of deletion, addition, insertion or substitution of one or more amino acids and showing a thermophilic glucoamylase activity.

Claims (9)

1. An isolated nucleic acid encoding a polypeptide having a thermostable glucoamylase activity selected from the group consisting of:

(a) a nucleic acid encoding a polypeptide having the amino acid sequence of SEQ ID NO:6 or an amino acid sequence that differs from SEQ ID NO:6 by one to up to three amino acid substitution(s) selected from the group consisting of:

i. substitution of Ser for Phe at position 187;

ii. substitution of Ile for Pro at position 123;

iii. substitution of Val for Leu at position 318;

(b) a nucleic acid having the nucleotide sequence of SEQ ID NO:7.

2. A recombinant DNA containing the nucleic acid defined by claim 1 .

3. A host cell transformed with the recombinant DNA defined by claim 2 .

4. A method for producing a polypeptide having a thermostable glucoamylase activity, the method comprising culturing the host cell defined by claim 3 and collecting a polypeptide having a thermostable glucoamylase activity from the culture.