Plant growth factor
View Patent ↗The present invention relates to a peptide of the formula: wherein R 1 and R 2 are the same or different and each represents SO 3 H or H; X represents an α-amino acid or a single bond; Z 1 and Z 2 are the same or different and each represents an α-amino acid; and Y represents OH or NH 2 . This peptide has plant growth factor properties.
1. An isolated plant growth factor peptide obtained by collecting cells from liliaceous plants, incubating the collected cells in a plant cell cultivation medium, and separating said plant growth factor peptide from the cells through centrifugation, wherein the peptide is either 4 or 5 amino acids long.
2. An isolated plant growth factor peptide, wherein the plant growth factor peptide is either 4 or 5 amino acids long and has the following physico-chemical properties:
a) it is soluble in water;
b) it is acidic;
c) it keeps 70% of its activity, after being heated at 100° C. for 10 minutes and it is inactivated, after being autoclaved at 121° C. for 20 minutes;
d) it is a polar substance, and is not retained in reversed-phase columns with porous spherical silica particles with an average size of 75 μm and an average pore size of 120 Å and a synthetic adsorbent ion exchange resin with the following chemical structure
e) it is stable at pH of 3 to 9, but at pH 11, its activity is reduced to 60%;
f) it is inactivated by a proteolytic enzyme obtained from Streptomyces griseus , but it is not inactivated by Glycosidases “Mixed”; and
g) it is adsorbed to 2-(diethylamino)ethyl-moiety containing ion-exchange resin (and eluted with 1000 mM KCl), but it is not adsorbed to a carboxymethyl-moiety containing ion-exchange resin.