PICA system detector calibration
View Patent ↗Methods, apparatus and data structures useful in correcting PICA image data are described. An exemplary method comprises acquiring optical image data of a target having identifiable optical-image features, acquiring PICA image data of the target having identifiable PICA-image features corresponding to the optical-image features, matching PICA-image features with corresponding optical-image features, and calculating from matched PICA-image features and optical-image features a set of coefficients defining relationships between observed positions of PICA-image features and optical-image features. Corrections are applied to the observed positions of detected photons based on the coefficients. The coefficients may provide a local correction using a bilinear relationship giving the transformation of a rectangle formed by four features of the PICA-image data to fit a corresponding rectangle in the optical-image data. Alternatively, the coefficients may provide a global mapping function defining transformation of any point of the PICA-image data to fit a corresponding point in the optical-image data.
1. A method of correcting PICA image data, comprising:
a. Acquiring optical image data of a target having identifiable optical-image features,
b. Acquiring PICA image data of the target having identifiable PICA-image features corresponding to the optical-image features,
c. Preparing optical-image-background data by applying gray-scale erosion to the optical-image data,
d. Subtracting optical-image-background data from the optical-image data to produce flattened-optical-image data have lesser intensity gradients than the optical-image data,
e. matching PICA-image features with corresponding features of the flattened-optical-image data, and
f. Calculating from matched PICA-image features and optical-image features a set of coefficients defining relationships between observed positions of PICA-image features and optical-image features.
2. An apparatus for preparing corrected PICA image data, comprising:
a. An optical microscope for acquiring optical image data of a target having identifiable optical-image features,
b. A PICA camera for acquiring PICA image data of the target having identifiable PICA-image features corresponding to the optical-image features, and
c. Data processing equipment having instructions for matching PICA-image features with corresponding optical-image features by,
Preparing optical-image-background data by applying gray-scale erosion to the optical-image data,
Subtracting optical-image-background data from the optical-image data to produce flattened-optical-image data having lesser intensity gradients than the optical-image data, and
Matching PICA-image features with corresponding features of the flattened-optical-image data,
and calculating from matched PICA-image features and optical-image features a set of coefficients defining relationships between observed positions of PICA-image features and optical-image features.