Compositions comprising reproductive cell media and methods for using such compositions
View Patent ↗Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian, avian, or piscian reproductive sperm cells. The compositions comprise a suitable reproductive cell media and a transforming growth factor, an insulin-like growth factor, or zinc, and, optionally, inositol, transferrin, or fructose, or combinations thereof.
1. A composition comprising a sperm cell and a medium, wherein the medium comprises at least one insulin-like growth factor and at least one transforming growth factor, and wherein the medium is a collection, holding, processing, in vitro fertilization, sexing, culturing or storage medium.
2. The composition of claim 1 , wherein the sperm cell is a mammalian sperm cell.
3. The composition of claim 2 , wherein the sperm cell is a porcine sperm cell.
4. The composition of claim 2 , wherein the sperm cell is an equine sperm cell.
5. The composition of claim 2 , wherein the sperm cell is a bovine sperm cell.
6. The composition of claim 2 , wherein the sperm cell is an ovine sperm cell.
7. The composition of claim 2 , wherein the sperm cell is a human sperm cell.
8. The composition of claim 1 , wherein the sperm cell is an avian sperm cell.
9. The composition of claim 1 , wherein the sperm cell is a piscian sperm cell.
10. The composition of claim 1 , wherein the transforming growth factor comprises TGFβ-1.
11. The composition of claim 1 , wherein the transforming growth factor comprises TGFβ-2.
12. The composition of claim 1 , wherein the transforming growth factor comprises TGFβ-1 and TGFβ-2.
13. The composition of claim 1 , wherein the insulin-like growth factor comprises IGFβ-1.
14. The composition of claim 12 , wherein the medium further comprises IGFβ-1.
15. The composition of claim 1 , wherein the medium further comprises at least one component selected from the group consisting of inositol, transferrin, and fructose.
16. The composition of claim 10 , wherein the medium is in liquid form and the TGFβ-1 is present in a concentration from about 0.1 ng/L to about 10 μL.
17. The composition of claim 16 , wherein the TGFβ-1 is present in a concentration from about 20 ng/L to about 400 ng/L.
18. The composition of claim 17 , wherein the TGFβ-1 is present in a concentration from about 50 ng/L to about 150 ng/L.
19. The composition of claim 11 , wherein the medium is in liquid form and the TGFβ-2 is present in a concentration from about 0.1 ng/L to about 200 ng/L.
20. The composition of claim 19 , wherein the TGFβ-2 is present in a concentration from about 0.4 ng/L to about 16 ng/L.
21. The composition of claim 19 wherein the TGFβ-2 is present in a concentration from about 1.8 ng/L to about 3.8 ng/L.
22. The composition of claim 13 , wherein the medium is in liquid form and the IGF-1 is present in a concentration from about 0.1 ng/L to about 30 μg/L.
23. The composition of claim 22 wherein the IGF-1 is present in a concentration from about 40 ng/L to about 640 ng/L.
24. The composition of claim 22 , wherein the IGF-1 is present in a concentration from about 200 ng/L to about 450 ng/L.
25. The composition of claim 1 , wherein the medium further comprises a cryopreservative.
26. The composition of claim 1 , wherein the medium further comprises zinc.
27. The composition of claim 3 , wherein the medium comprises TGFβ-1, TGFβ-2, and IGF-1.