IP Library › Granted Patent US 7,399,605
Granted Patent B2
US 7,399,605 · App. 10/085,476 · Granted Jul 15, 2008

Method for identifying a HCV RNA-dependent RNA polymerase inhibitor

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Quick Facts
Patent No.
US 7,399,605
App. No.
10/085,476
Granted
Jul 15, 2008
Kind
B2
Abstract

The present invention relates to the molecular biology and virology of the hepatitis C virus (HCV). An object of the present invention is a method to reproduce in vitro the RNA-dependent RNA polymerase activity of HCV that makes use of sequences contained in the HCV NS5B protein.

Claims (4)

1. A method for identifying a HCV RNA-dependent RNA polymerase inhibitor comprising:

(a) incubating in vitro a composition comprising a purified HCV NS5B recombinant protein, ribonucleotide substrates, an RNA template, and a test compound, under conditions suitable to produce NS5B RNA-dependent RNA polymerase activity in the absence of said test compound, wherein said recombinant protein was expressed in either a eukaryotic or prokaryotic heterologous system and purified to apparent homogeneity, wherein said NS5B is the only HCV protein present during said incubating; and

(b) measuring the ability of said test compound to inhibit said NS5B RNA-dependent RNA polymerase activity and thereby identifying an HCV RNA-dependent RNA polymerase inhibitor.

2. The method of claim 1 , wherein said method measures primer independent RNA-dependent RNA polymerase activity.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 21, 2014
From: ISTITUTO DI RICERCHE DI BIOLOGIA MOLECOLARE P. ANGELETTI S.P.A.
To: MSD ITALIA S.R.L.
Reel/Frame 032720/0243 →