Transaldolase gene
View Patent ↗Attempts are made to provide a novel transaldolase gene; a polypeptide encoded by this gene; a recombinant DNA obtained by integrating this gene; a microorganism carrying this recombinant DNA; and a process for producing an aromatic amino acid, an aromatic vitamin, L-histidine, riboflavin, a nucleic acid, a nucleic acid-associated substance, a novel saccharide, etc. by using the above microorganism. As the results of extensive studies, a novel transaldolase gene is isolated from chromosomal DNA of a microorganism belonging to the genus Corynebacterium as a DNA fragment complementary to the requirement for shikimic acid of a transketolase defective variant obtained as a variant with the requirement for shikimic acid belonging to the genus Corynebacterium . Further, a recombinant DNA containing this gene is constructed and transferred into a host microorganism, thereby achieving the objects as described above.
1. An isolated DNA coding for a polypeptide comprising the amino acid sequence of SEQ ID NO:4.
2. An isolated DNA comprising the nucleotide sequence of SEQ ID NO: 2.
3. A recombinant DNA obtained by ligating the DNA of claim 1 or 2 with a vector.
4. An isolated host cell transformed with the recombinant DNA of claim 3 .
5. A process for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:4, said process comprising the steps of:
culturing the transformed of claim 4 in a medium to thereby produce and accumulate the polypeptide in culture, and
recovering the polypeptide from the culture.