IP Library Granted Patent US 7,018,795
Granted Patent B2
US 7,018,795 · App. 10/103,830 · Granted Mar 28, 2006

Hybridization probe and target nucleic acid detecting kit, target nucleic acid detecting apparatus and target nucleic acid detecting method using the same

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Quick Facts
Patent No.
US 7,018,795
App. No.
10/103,830
Granted
Mar 28, 2006
Kind
B2
Abstract

The present invention relates to a hybridization probe having a rod-shaped boby and nucleic acid which is bonded to the rod-shape material and specifically bonds to a target nucleic acid and also relates to a target nucleic detecting kit, a target nucleic acid detecting apparatus and a target nucleic acid detecting method using the same.

Claims (20)

1. A hybridization probe comprising,

a helical organic molecule having a length of 810 nm or shorter which is aligned to form a film, wherein said helical organic molecule is not DNA; and

a polynucleotide bonded to the helical organic molecule wherein said polynucleotide hybridizes to a target polynucleotide, and

wherein the film reflects incident light as a colored interference light, thereby allowing detection of the target polynucleotide by detecting a change in color of the colored interference light.

2. The hybridization probe according to claim 1 , wherein the polynucleotide is RNA or single-stranded DNA.

3. The hybridization probe according to claim 1 , wherein the target polynucleotide is selected from the group consisting of:

a) a prokaryotic polynucleotide;

b) a eukaryotic, non-human polynucleotide; and

c) a polynucleotide that exists only in a human.

4. The hybridization probe according to claim 1 , wherein the target polynucleotide is selected from the group consisting of a cancer-related gene, a gene related to a genetic disease, a virogene, a bacterial gene and a gene having a polymorphism that is a risk factor for a disease.

5. The hybridization probe according to claim 1 , wherein presence of the target polynucleotide indicates a presence of a second target polynucleotide.

6. The hybridization probe according to claim 1 , wherein the hybridization probe is amphiphilic.

7. The hybridization probe according to claim 1 , wherein the helical organic molecule is selected from the group consisting of an α-helix polypeptide and amylase.

8. The hybridization probe according to claim 7 , wherein the hybridization probe is amphiphilic.

9. The hybridization probe according to claim 8 , wherein the helical organic molecule is a block polymer of α-helix polypeptide.

10. The hybridization probe according to claim 1 , wherein the hybridization probe is amphiphilic.

11. A target polynucleotide detecting kit comprising:

a hybridization probe; and

any one of a dish, plate or tube;

wherein the hybridization probe comprises a helical organic molecule, and a polynucleotide bonded to the helical organic molecule; wherein the helical organic molecule is not DNA, and wherein the helical organic molecule has a length of 810 nm or shorter which is aligned to form a film, wherein the polynucleotide hybridizes to a target polynucleotide, and wherein the film reflects incident light as colored interference light, thereby allowing detection of the target polynucleotide by detecting a change in color of the colored interference light.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 15, 2007
From: FUJIFILM HOLDINGS CORPORATION (FORMERLY FUJI PHOTO FILM CO., LTD.)
To: FUJIFILM CORPORATION
Reel/Frame 018904/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 12, 2002
From: KINOSHITA, TAKATOSHI; WASHIZU, SHINTARO
To: FUJI PHOTO FILM CO., LTD
Reel/Frame 012992/0669 →