Root-specific, stimulant inducible promoter and its use
View Patent ↗This invention relates to an isolated isoflavone synthase 1 (IFS1) promoter nucleic acid fragment. The invention also relates to the construction of chimeric genes comprising all or a portion of the IFS1 promoter directing the expression of transgenes, in sense or antisense orientation.
1. An isolated polynucleotide comprising a promoter having the nucleotide sequence of SEQ ID NO: 17 operably linked to a nucleotide fragment, wherein expression of said nucleotide fragment by the promoter is root specific.
2. A recombinant construct comprising the isolated polynucleotide of claim 1 .
3. A plant comprising the recombinant construct of claim 2 .
4. The plant of claim 3 wherein said plant is selected from the group consisting of a monocot and a dicot.
5. The plant of claim 3 wherein said plant is a monocot selected from the group consisting of corn, rice, wheat, barley and palm.
6. The plant of claim 3 wherein said plant is a dicot selected from the group consisting of Arabidopsis , soybean, oilseed Brassica , peanut, sunflower, safflower, cotton, tobacco, tomato, potato, and cocoa.
7. The plant of claim 6 wherein said plant is soybean.
8. Transgenic grain from the plant of claim 3 comprising the recombinant construct of claim 2 .
9. A method of expressing a polypeptide in root cells comprising:
(a) transforming a plant cell with the recombinant construct of claim 2 , wherein the nucleotide fragment comprises an exogenous coding region coding for a polypeptide;
(b) selecting for a transformed plant cell of (a); and
(c) regenerating a stably transformed plant from the transformed plant cell of (b), wherein expression of the nucleotide fragment results in expression of the polypeptide in root cells.
10. The method of claim 9 wherein said plant is selected from the group consisting of a monocot and a dicot.
11. The method of claim 9 wherein the plant is a monocot selected from the group consisting of corn, rice, wheat, barley and palm.
12. The method of claim 9 wherein the plant is a dicot selected from the group consisting of Arabidopsis , soybean, oilseed Brassica , peanut, sunflower, safflower, cotton, tobacco, tomato, potato, and cocoa.
13. The method of claim 12 wherein the plant is soybean.
14. A method of expressing a nucleotide fragment in the root of a plant comprising:
(a) transforming a plant cell with the recombinant construct of claim 2 ;
(b) selecting for a transformed plant cell of (a); and
(c) regenerating a stably transformed plant from the transformed plant cell of (b), wherein the nucleotide fragment is expressed in the root of the transformed plant.