Cell-containing bone graft material

1. A musculoskeletogenic (MSG) graft composite made from whole bone marrow aspirate (BMA), the graft composite consisting of:

a) a physiologic fraction obtained from centrifugation of whole BMA, consisting essentially of:

i) musculoskeletal progenitor cells (MSPCs) at a level greater than their native level in whole BMA, and

ii) red blood cells (RBCs) derived from BMA at a level less than their native level in whole BMA,

iii) platelets at a level greater than their native level in whole BMA, and

b) a porous sterile matrix having an D 50 average pore size of at least 20 μm,

c) a platelet release agent added in an amount sufficient to cause the release of growth factors from BMA-derived platelets,

wherein the (a) physiologic fraction of BMA and the (c) platelet release agent are contained within the (b) porous sterile matrix, thereby causing release of growth factors from the BMA-derived platelets,

wherein the matrix is demineralized bone matrix and the platelet release agent is thrombin.

2. The composite of claim 1 wherein the MSPCs are present in the physiologic fraction at a level 2 times greater than their native level in whole BMA.

3. The composite of claim 1 wherein the MSPCs are present in the physiologic fraction at a level 5 times greater than their native level in whole BMA.

4. The composite of claim 1 wherein the RBCs derived from BMA are present in the physiologic fraction at a level less than 20% their native level in whole BMA.

5. The composite of claim 1 wherein the porous matrix has a D 50 average pore size of at least 50 μm.

6. The composite of claim 1 wherein the porous matrix has a D 50 average pore size of at least 100 μm.

7. The composite of claim 1 wherein the ratio of the physiologic fraction to the matrix is between 1:1 (v/v) and 1:20 (v/v).