Process for microbial production of ubiquinone-10 using
View Patent ↗The present invention provides a process for producing ubiquinone-10 using a microorganism having the ability to form ubiquinone-10 and having one or more properties selected from the group consisting of the property wherein geranylgeranyltransferase activity is reduced or defective, the property wherein decaprenyldiphosphate synthetase activity is strengthened, and the property wherein p-hydroxybenzoic acid-decaprenyltransferase activity is strengthened, DNA and a polypeptide useful for the production process, microorganisms useful for the production, a method for expressing a gene in the microorganisms, and a method for breeding the microorganisms.
1. A process for producing ubiquinone-10, which comprises:
obtaining a microorganism belonging to the species Rhodobacter sphaeroides having the ability to form ubiquinone-10 and having a disrupted chromosomal geranylgeranyl pyrophosphate synthetase gene, wherein geranylgeranyl pyrophosphate synthetase activity is defective;
transforming the microorganism with a DNA selected from the group consisting of:
(a) a DNA encoding a polypeptide comprising an amino acid sequence having at least 95% homology to the amino acid sequence shown in SEQ ID NO:2 and having decaprenyldiphosphate synthetase activity, and
(b) a DNA encoding a polypeptide comprising an amino acid sequence having at least 95% homology to the amino acid sequence shown in SEQ ID NO:4 and having p-hydroxybenzoic acid-decaprenyltransferase activity;
culturing the transformed microorganism in culture;
allowing ubiquinone-10 to form and accumulate in the culture; and
recovering ubiquinone-10 from the culture.
2. The process according to claim 1 , wherein the chromosomal geranylgeranyl pyrophosphate synthetase gene is a DNA comprising the nucleotide sequence of SEQ ID NO: 6.
3. A process according to claim 1 or 2 , wherein the disrupted chromosomal geranylgeranyl pyrophosphate synthetase gene is obtained by homologous recombination.