Genomic mammary Amyloid a sequence
View Patent ↗A genomic nucleotide sequence encoding Serum Amyloid A (SAA), isolated and purified from mammalian colostrum, is disclosed. Methods of use for the same in transgenic protocols is also disclosed.
1. A purified and isolated nucleic acid molecule that encodes colostrum associated serum amyloid A (SAA) protein comprising the TFLK amino acid sequence depicted in SEQ ID NO: 34, wherein said nucleic acid molecule comprises one or more non-coding regions from SEQ ID NO: 31.
2. The nucleic acid molecule of claim 1 wherein said molecule is purified and isolated from bovine species.
3. The nucleic acid molecule of claim 1 wherein said non coding region is 5′ of base 648 in SEQ ID NO: 31.
4. The nucleic acid molecule of claim 1 wherein said non-coding region consists of bases 739 to 2982 of SEQ ID NO: 31 or the complement thereof.
5. The nucleic acid molecule of claim 1 wherein said non-coding region consists of bases 3122 to 3510 of SEQ ID NO: 31 or the complement thereof.
6. The nucleic acid molecule of claim 1 wherein said non-coding region consists of bases 3674 to 4984 of SEQ ID NO: 31 or the complement thereof.
7. An expression cassette which comprises the nucleic acid molecule of claim 1 operably linked to a promoter region.
8. A cloning or expression vector comprising the expression cassette of claim 7 .
9. An expression cassette which comprises the nucleic acid molecule of claim 8 operably linked to a promoter nucleotide.
10. The nucleic acid molecule of claim 1 wherein said TFLK amino acid sequence depicted in SEQ ID NO: 34 is located within amino acids 1–26 at the N-terminal region of said protein.
11. A purified and isolated nucleic acid molecule that encodes colostrum associated serum amyloid A (SAA) protein comprising the TFLK amino acid sequence depicted in SEQ ID NO: 34 , wherein said nucleic acid molecule encodes the peptide of SEQ ID NO:3 or SEQ ID NO: 4 and one or more of a sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6,SEQ ID NO: 7 and SEQ ID NO: 8.
12. A purified and isolated genomic bovine nucleotide sequence which encodes colostrum associated serum amyloid A (SAA) protein said sequence comprising a non-coding region selected from the group consisting of bases 1–647, 739–2982, 3122–3510, 3674–4984 of SEQ ID NO: 31 or the complement thereof.
13. A eucaryotic or procaryotic host cell transformed with a vector comprising the nucleotide sequence of claim 12 .
14. A purified and isolated nucleic acid molecule that encodes colostrum associated serum amyloid A (SAA) comprising the TFLK amino acid sequence depicted in SEQ ID NO:34 , wherein said nucleic acid molecule comprises SEQ ID NO: 31.
15. A eucaryotic or procaryotic host cell transformed with a vector comprising the nucleic acid molecule of claim 14 .
16. The nucleic acid molecule of claim 14 wherein said TFLK amino acid sequence depicted in SEQ ID NO: 34 is located within amino acids 1–26 at the N-terminal region of said protein.
17. A method for producing mammary-associated serum amyloid A 3 (MAA) peptide comprising:
administering a MAA inducing agent to a bovine mammary epithelial cell line;
culturing said cell so that MAA is produced;
harvesting said MAA from said mammary epithelial cell; and
purifying said MAA from said cell.
18. A bovine mammary-associated serum amyloid A 3 (MAA) peptide produced by the method of claim 17 .
19. A method for producing mammary-associated serum amyloid A 3 (MAA) peptide comprising:
administering a MAA inducing agent to a bovine mammary epithelial cell line;
culturing said cell so that MAA is secreted from said cell;
harvesting said MAA from said mammary epithelial cell culture; and
purifying said MAA from said cell culture.
20. A bovine mammary-associated serum amyloid A 3 (MAA) peptide produced by the method of claim 19 .