Probes for detecting tumor cells
Probes are provided that are useful for detecting CK19 target sequence in a test sample.
1. A composition of matter for detecting a CK19 target sequence comprising a nucleic acid selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7, the complements of SEQ ID NO: 6, and the complement of SEQ ID NO: 7.
2. The composition of matter of claim 1 , wherein the nucleic acid is selected from the group consisting of SEQ ID NO: 6 and SEQ ID NO: 7.
3. The composition of matter of claim 2 , further comprising a fluorescent label and a fluorescence quencher.
4. A method of amplifying a CK19 target sequence comprising the steps of:
(a) forming a reaction mixture comprising nucleic acid amplification reagents, the composition of matter of claim 1 , and a test sample suspected of containing a target sequence; and
(b) subjecting the mixture to amplification conditions to generate at least one copy of a nucleic acid sequence complementary to the target sequence.
5. A kit for amplifying a CK19 target sequence comprising:
(a) the composition of matter of claim 1 ; and
(b) amplification reagents.