Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair
View Patent ↗Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed.
1. A method of treating a cartilage defect in a mammal, the method comprising:
a) differentiating an isolated human adipose tissue derived stromal cell into a chondrocytic cell; and
b) administering said chondrocytic cell to a cartilage defect area in a mammal.
2. The method of claim 1 , wherein said cell further comprises a biocompatible matrix.
3. The method of claim 2 , wherein said biocompatible matrix is selected from the group consisting of calcium alginate, agarose, fibrin, an collagen.
4. The method of claim 1 , wherein said adipose tissue derived stromal cell is genetically modified.
5. The method of claim 1 , wherein said mammal is a human.
6. The method of claim 1 , wherein following administration of said chondrocytic cell to a cartilage defect area in a mammal, the cell further differentiates in situ in said human.