Transgenic high tryptophan plants
View Patent ↗The present invention provides transgenic plants transformed with an isolated DNA encoding a monomeric anthranilate synthase. The present invention also provides an isolated DNA encoding a monomeric anthranilate synthase from Agrobacterium tumefaciens . Transformation vectors and transformed plants containing the isolated DNA and seeds derived therefrom, are also provided.
1. An isolated DNA encoding a monomeric anthranilate synthase, wherein the isolated DNA is selected from the group consisting of (a) a DNA sequence encoding a monomeric anthranilate synthase comprising at least 95% identity to SEQ ID NO:4; (b) a DNA sequence that hybridizes to SEQ ID NO:1 under stringent conditions; and (c) a DNA sequence that hybridizes to SEQ ID NO:75 under stringent conditions; wherein the stringent conditions include a wash at 50° C. in 0.015 M NaCl/0.0015 M sodium citrate (SSC) and 0.1% sodium lauryl sulfate (SLS).
2. The isolated DNA of claim 1 , wherein the isolated DNA comprises SEQ ID NO:1.
3. The isolated DNA of claim 1 , wherein the isolated DNA further encodes a plastid transit peptide.
4. The isolated DNA of claim 3 , wherein the plastid transit peptide comprises SEQ ID NO:72 or 74.
5. The isolated DNA of claim 1 , wherein the isolated DNA further encodes a selectable marker gene or a reporter gene.
6. The isolated DNA of claim 5 , wherein the selectable marker gene, when expressed in a plant, imparts herbicide resistance to cells of said plant.
7. The isolated DNA of claim 6 , wherein the herbicide resistance comprises resistance to glyphosate, glufosinate or dalapon.
8. The isolated DNA of claim 1 , wherein the isolated DNA further encodes a Bacillus thuringiensis protein that, when expressed in a plant, imparts insect resistance to the plant.
9. The isolated DNA of claim 1 , wherein the isolated DNA encoding the anthranilate synthase comprises a promoter operably linked thereto.
10. A vector comprising the isolated DNA of claim 1 .
11. A seed comprising the isolated DNA of claim 1 .
12. A transgenic plant comprising the isolated DNA of claim 1 encoding a monomeric anthranilate synthase operably linked to a promoter.
13. The transgenic plant of claim 12 , wherein expression of the monomeric anthranilate synthase elevates the level of L-tryptophan in the plant relative to a second plant of the same or similar genetic background that does not include the transgene.
14. The transgenic plant of claim 12 , wherein the isolated DNA encodes SEQ ID NO:4.
15. The transgenic plant of claim 12 , wherein the isolated DNA further comprises a DNA encoding a plastid transit peptide.
16. The transgenic plant of claim 15 , wherein the plastid transit peptide comprises SEQ ID NO:72 or 74.
17. The transgenic plant of claim 12 , wherein the isolated DNA further encodes a selectable marker gene or a reporter gene.
18. The transgenic plant of claim 17 , wherein the selectable marker gene, when expressed in a plant, imparts herbicide resistance to cells of said plant.
19. The transgenic plant of claim 18 , wherein the herbicide resistance comprises resistance to glyphosate, glufosinate or dalapon.
20. The transgenic plant of claim 12 , wherein the isolated DNA further encodes a Bacillus thuringiensis protein that, when expressed in a plant, imparts insect resistance to the plant.
21. The transgenic plant of claim 12 , wherein the plant is a dicot.
22. The transgenic plant of claim 21 , wherein the plant is soybean or canola.
23. The transgenic plant of claim 12 , wherein the plant is a monocot.
24. The tranagenic plant of claim 23 , wherein the plant is maize, rice, wheat, barley or sorghum.
25. A seed of the transgenic plant of claim 12 , wherein the seed contains the isolated DNA encoding the monomeric anthranilate synthase.
26. The transgenic plant of claim 12 , wherein the monomeric anthranilate synthase comprises at least 95% identity to SEQ ID NO:4.
27. The transgenic plant of claim 12 , wherein the isolated DNA comprises SEQ ID NO:1.
28. The transgenic plant of claim 26 , wherein the isolated DNA further comprises a DNA encoding a plastid transit peptide.
29. The transgenic plant of claim 28 , wherein the plastid transit peptide comprises SEQ ID NO:72 or 74.
30. The transgenic plant of claim 26 , wherein the isolated DNA further encodes a selectable marker gene or a reporter gene.
31. The transgenic plant of claim 30 , wherein the selectable marker gene, when expressed in a plant, imparts herbicide resistance to cells of said plant.
32. The transgenic plant of claim 31 , wherein the herbicide resistance comprises resistance to glyphosate, glufosinate or dalapon.
33. The transgenic plant of claim 26 , wherein the isolated DNA further encodes a Bacillus thuringiensis protein that, when expressed in a plant, imparts insect resistance to the plant.
34. The transgene plant of claim 26 , wherein the plant is a dicot.
35. The transgenic plant of claim 34 , wherein the plant is soybean or canola.
36. The transgenic plant of claim 26 , wherein the plant is a monocot.
37. The transgenic plant of claim 34 , wherein the plant is maize, rice, wheat, barley or sorghum.
38. A seed of the transgenic plant of claim 26 wherein the seed contains the isolated DNA.
39. The isolated DNA of claim 1 , wherein the isolated DNA encodes a monomeric anthranilate synthase comprising at least 95% identity to SEQ ID NO:4.
40. The isolated DNA of claim 1 , wherein the isolated DNA comprises a DNA sequence that hybridizes to SEQ ID NO:1 under stringent conditions, wherein the stringent conditions include a wash at 50° C. in 0.015 M NaCl/0.0015 M sodium citrate (SSC) and 0.1% sodium lauryl sulfate (SLS).
41. The isolated DNA of claim 1 , wherein the isolated DNA comprises a DNA sequence that hybridizes to SEQ ID NO:75 under stringent conditions, wherein the stringent conditions include a wash at 50° C. in 0.015 M NaCl/0.0015 M sodium citrate (SSC) and 0.1% sodium lauryl sulfate (SLS).
42. The transgenic plant of claim 12 , wherein the isolated DNA comprises a DNA sequence that hybridizes to SEQ ID NO:1 under stringent conditions, wherein the stringent conditions include a wash at 50° C. in 0.015 M NaCl/0.0015 M sodium citrate (SSC) and 0.1% sodium lauryl sulfate (SLS).
43. The transgenic plant of claim 12 , wherein the isolated DNA comprises a DNA sequence that hybridizes to SEQ ID NO:75 under stringent conditions, wherein the stringent conditions include a wash at 50° C. in 0.015 M NaCl/0.0015 M sodium citrate (SSC) and 0.1% sodium lauryl sulfate (SLS).