Design of artificial genes for use as controls in gene expression analysis systems
View Patent ↗Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.
1. A control for use in a gene expression analysis system comprising:
a known amount of at least one DNA target generated from at least one intergenic or intronic region of genomic DNA from a known sequence; or
a known amount of at least one spike mRNA generated from DNA generated from said at least one intergenic or intronic region of genomic DNA from a known sequence, wherein
(a) said at least one DNA is selected from the group consisting of
(i) SEQ ID No: 1; and
(ii) a complete complement of the sequence set forth in (i); or
(b) said at least one mRNA is transcribed from the group consisting of
(i) SEQ ID No: 11; and
(ii) a complete complement of the sequence set forth in (i).
2. The control of claim 1 wherein, said at least one DNA target or spike mRNA do not hybridize at high stringency with any DNA or mRNA from a source other than said at least one intergenic or intronic region of genomic DNA.
3. The control of claim 1 , wherein said at least one DNA target or spike mRNA is formulated at selected concentrations and ratios.
4. The control of claim 1 , wherein said at least one DNA target is addressably disposed upon a substrate.
5. The control of claim 1 , wherein said at least one spike mRNA is detectably labeled.