Electrochemical methods and devices for use in the determination of hematocrit corrected analyte concentrations
View Patent ↗Methods and devices for determining the concentration of an analyte in a physiological sample are provided which correct for errors in the analyte concentration measurement which are due to the hematocrit of the sample.
1. A method for determining the concentration of an analyte in a physiological sample, said method comprising:
(a) introducing said physiological sample into an electrochemical cell comprising:
(i) spaced apart working and reference electrodes; and
(ii) a redox reagent system comprising an enzyme and a mediator;
(b) applying a first electric potential having a first polarity to said reaction cell and measuring cell current as a function of time to obtain a first time-current transient;
(c) applying a second electric potential having a second polarity to said cell, wherein said second polarity is opposite said first polarity, and measuring cell current as a function of time to obtain a second time-current transient;
(d) deriving a preliminary analyte concentration from said first and second time-current transients; and
(e) multiplying said preliminary analyte concentration less a background value by a correction factor to derive said corrected analyte concentration in said sample;
whereby the corrected concentration of said analyte in said sample is determined.
2. The method according to claim 1 , wherein said physiological sample is whole blood or a derivative thereof.
3. The method according to claim 1 , wherein said analyte is glucose.