Exchangeable flow cell assembly with a suspended capillary
View Patent ↗There is provided a flow cell assembly in which a shuttle supports and positions a capillary with its end extending beyond the shuttle. The flow cell assembly facilitates the replacement of a flow cell which is damaged or with flow cells having capillaries of different size or shape.
1. A flow cytometer comprising
an exchangeable flow cell having a flow cell body and a capillary carried by and having one end extending beyond said flow cell body, wherein said flow cell body is provided with a recess extending along a portion of the length of the body, and includes means for accurately positioning the capillary in the recess along the length of the flow cell body,
a block having an opening adapted to receive the exchangeable flow cell and being at least partially formed by two flat surfaces, wherein said positioning means engages said flat surfaces to position the capillary relative to the block, and said block includes means for urging the flow cell body against the flat surfaces, and
a light source for emitting light to illuminate a portion of the capillary.
2. The flow cytometer of claim 1 , wherein said flow cell body has an aperture in the recess for permitting light from said light source to illuminate a portion of the capillary.
3. The flow cytometer of claim 1 , further comprising a pump for drawing a sample through the capillary.
4. The flow cytometer of claim 3 further comprising means for connecting said capillary to said pump.
5. The flow cytometer of claim 1 , further comprising a pump for drawing a sample through the capillary.
6. The flow cytometer of claim 5 further comprising means for connecting said capillary to said pump.
7. The flow cytometer of claim 1 wherein the positioning means comprises spaced members which engage the capillary at spaced positions along the capillary.
8. The flow cytometer of claim 7 wherein said spaced members are provided on inside faces of said elongated recess and said capillary engages said members to be accurately positioned in said flow cell body.
9. The flow cytometer of claim 8 wherein said spaced members comprise crossed pins.
10. The flow cytometer of claim 1 wherein the capillary includes a mask having a slit through which light passes.
11. The flow cytometer of claim 10 wherein the mask is formed on the entrance wall of a rectangular capillary and a mask having spaced slits is formed on the opposite wall to pass light scattered by a particle or cell and block light traveling directly from the entrance slit.
12. The flow cytometer of claim 11 wherein the mask is formed on the entrance wall of a rectangular capillary and masks are formed on the side wall with slits which coincide with the entrance slit whereby fluorescent light emitted by a particle in the light beam travels through the slits for detection.