Nucleic acids encoding PRO4400 polypeptides
View Patent ↗The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
1. An isolated nucleic acid comprising:
(a) the nucleic acid sequence of SEQ ID NO:131 or the complement thereof;
(b) the full-length coding sequence of the nucleic acid sequence of SEQ ID NO:131 or the complement thereof; or
(c) the full-length coding sequence of the cDNA deposited under ATCC accession number 203963 or the complement thereof.
2. The isolated nucleic acid of claim 1 comprising the nucleic acid sequence of SEQ ID NO:131 or the complement thereof.
3. The isolated nucleic acid of claim 1 comprising the full-length coding sequence of the nucleic acid sequence of SEQ ID NO:131 or the complement thereof.
4. The isolated nucleic acid of claim 1 comprising the full-length coding sequence of the cDNA deposited under ATCC accession number 203963 or the complement thereof.
5. A vector comprising the nucleic acid of claim 1 .
6. The vector of claim 5 , wherein said nucleic acid is operably linked to control sequences recognized by a host cell transformed with the vector.
7. An isolated host cell comprising the vector of claim 5 .
8. The host cell of claim 7 , wherein said cell is a CHO cell, an E. coli or a yeast cell.
9. An isolated nucleic acid molecule consisting of an at least 30 nucleotide fragment of the nucleic acid sequence of SEQ ID NO:131, or the complement thereof, that specifically hybridizes under stringent conditions to:
(a) the nucleic acid sequence of SEQ ID NO:131 or the complement thereof;
(b) the full-length coding sequence of the cDNA deposited under ATCC accession number 203963 or the complement thereof;
wherein, said stringent conditions use 50% formamide, 5×SSC, 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 50% formamide at 55° C., followed by a wash comprising of 0.1×SSC containing EDTA at 55° C., wherein said isolated nucleic acid molecule is suitable for use as a primer or probe.
10. The isolated nucleic acid molecule of claim 9 that is at least 50 nucleotides in length.
11. The isolated nucleic acid molecule of claim 9 that is at least 60 nucleotides in length.
12. The isolated nucleic acid molecule of claim 9 that is at least 70 nucleotides in length.
13. The isolated nucleic acid molecule of claim 9 that is at least 80 nucleotides in length.
14. The isolated nucleic acid molecule of claim 9 that is at least 90 nucleotides in length.
15. The isolated nucleic acid molecule of claim 9 that is at least 100 nucleotides in length.