Stimulable phosphor sheet for detection of substances originating from living body or its analogues
View Patent ↗A method employing a porous material sheet which has at predetermined positions plural dots each being composed of a group of probe molecules and a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m 2 is favorably utilized to detect by autoradiography radioactively labeled substances originating from living body or its analogues which are able to be fixed to the probe molecules by biochemically specific binding reaction.
1. A process for detecting complementary nucleic acid fragments which comprises the steps of:
preparing a composite material sheet, wherein said composite material sheet comprises partitions two-dimensionally extending on a sheet plane to form plural fine sections on the sheet plane, and a porous material portion placed in each fine section, wherein a group of probe molecules are attached to each porous material portion;
bringing radioactively labeled sample substances in a liquid phase, into contact with the prepared composite material sheet, whereby target substances among the sample substances are fixed to the probe molecules by a specific biochemical reaction;
removing unfixed sample substances from the composite material sheet;
keeping the composite material sheet having sample substances fixed by a specific biochemical reaction in contact with a stimulable phosphor sheet, wherein the stimulable phosphor sheet has a phosphor layer which has a thickness of 5 to 50 μm and which contains a stimulable phosphor in an amount of 10 to 140 g/m 2 , whereby the stimulable phosphor layer absorbs and stores radiation energy of the radioactive label coming from the fixed target substances;
irradiating the stimulable phosphor sheet with a stimulating light, whereby the phosphor layer in which the radiation energy is stored releases stimulated emissions;
detecting the stimulated emissions photoelectrically to obtain a series of electric signals; and
processing the electric signals to detect dots in which the target substances are present.
2. The process of claim 1 , wherein the probe molecules are nucleic acid fragments, and the target substances are nucleic acid fragments complementary to the probe molecules.
3. The process of claim 1 , wherein the probe molecules are single-stranded nucleic acid fragments and the target substances in the sample substances are single-stranded nucleic acid fragments complementary to the probe molecules.
4. The process of claim 1 , wherein the partitions of the composite material sheet are made of material having a mean density of not lower than 0.6 g/cm 3 and the porous material portions have a mean density of not higher than 1.0 g/cm 3 , provided that the mean density of material of the partitions is higher than the mean density of the material of the porous material portions.
5. The process of clam 1 , wherein at least one of an upper and a lower surface of the porous material portions in the composite material sheet retracts from an upper or a lower surface of adjoining partitions on the sheet plane.