siRNA treatment of diseases or conditions related to levels of IKK-gamma
View Patent ↗The present invention relates to nucleic acid molecules, including antisense and enzymatic nucleic acid molecules, such as hammerhead ribozymes, DNAzymes, allozymes, aptamers, decoys and siRNA (RNAi), which modulate the expression or function of IKK genes, such as IKK-gamma, IKK-alpha, or IKK-beta, and PKR genes.
1. A chemically modified double stranded siRNA molecule that down regulates expression of an IKK-gamma gene via RNA interference (RNAi), wherein:
a) each strand of said siRNA molecule is independently about 18 to about 28 nucleotides in length; and
b) one strand of said siRNA molecule comprises nucleotide sequence having sufficient complementarity to an RNA of said IKK-gamma gene for the siRNA molecule to direct cleavage of said RNA via RNA interference.
2. The siRNA molecule of claim 1 , wherein each strand of the siRNA molecule comprises about 18 to about 28 nucleotides, and wherein each strand comprises at least about 14 to 24 nucleotides that are complementary to the nucleotides of the other strand.
3. The siRNA molecule of claim 1 , wherein said siNA molecule is assembled from two separate oligonucleotide fragments wherein a first fragment comprises the sense strand and a second fragment comprises the antisense strand of said siNA molecule.
4. The siRNA molecule of claim 3 , wherein said sense strand is connected to the antisense strand via a linker molecule.
5. The siRNA molecule of claim 4 , wherein said linker molecule is a polynucleotide linker.
6. The siRNA molecule of claim 4 , wherein said linker molecule is a non-nucleotide linker.
7. The siRNA molecule of claim 3 , wherein said second fragment comprises a terminal cap moiety at a 5′-end, a 3′-end, or both of the 5′ and 3′ ends of said second strand.
8. The siRNA molecule of claim 7 , wherein said terminal cap moiety is an inverted deoxy abasic moiety.
9. The siRNA molecule of claim 3 , wherein said first fragment comprises a phosphorothioate internucleotide linkage at the 3′ end of said first strand.
10. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises at least one 2′-sugar modification.
11. The siRNA molecule of claim 10 , wherein said 2′-sugar modification is a 2′-deoxy-2′-fluoro modification.
12. The siRNA molecule of claim 10 , wherein said 2′-sugar modification is a 2′-O-methyl modification.
13. The siRNA molecule of claim 10 , wherein said 2′-sugar modification is a 2′-deoxy modification.
14. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises at least one nucleic acid base modification.
15. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises at least one phosphate backbone modification.
16. A composition comprising the siRNA molecule of claim 1 in a pharmaceutically acceptable carrier or diluent.