Salutaridinol 7-O-acetyltransferase and derivatives thereof
View Patent ↗This invention provides: (A) a protein the sequence of which is in FIG. 8 (SEQ. ID n° 14), or a fragment thereof having at least 15 amino acids, or a variant thereof, having at least 70% identity with FIG. 8 (SEQ. ID n° 14) over a length of at least 400 amino acids; (B) a peptide comprising a fragment of salutaridinol 7-O-acetyltransferase of at least 6 consecutive amino acids not present in other plant acetyltransferases as in FIG. 2 (SEQ. IDs n° 8 to 12); (C) a nucleic acid (i) the sequence of which is in FIG. 9 (SEQ. ID n° 13) or FIG. 10 (SEQ. ID n° 15), or (ii) a fragment thereof of at least 45 nucleotides, or (iii) a variant thereof, having at least 70% identity with FIG. 9 (SEQ. ID n° 13) or FIG. 10 (SEQ. ID n° 15) over a length of at least 1200 bases, or (iv) a sequence complementary to (i), (ii) or (iii); or (v) the RNA equivalent of any of (i), (ii), or (iii); (D) a nucleic acid comprising a fragment of a salutaridinol 7-O-acetyltransferase gene of at least 18 consecutive nucleotides unique to the salutaridinol 7-O-acetyltransferase gene from the 5′ or 3′ untranslated regions in FIG. 9 (SEQ. ID n° 13), or a sequence complementary thereto. This invention also provides methods for producing pentacyclic morphinan alkaloids and thebaine.
1. An isolated nucleic acid comprising the coding sequence of the molecule SAT 2 CO48 illustrated in FIG. 11 (SEQ. ID No. 17).
2. An isolated nucleic acid encoding a protein comprising consecutive amino acids, the amino acid sequence of which comprises the amino acid sequence illustrated in FIG. 8 (SEQ. ID No. 14).
3. An isolated nucleic acid comprising consecutive nucleotides, the nucleotide sequence of which comprises the nucleic acid sequence illustrated in FIG. 9 (SEQ. ID No. 13) or FIG. 10 (SEQ. ID No. 15).
4. A method for producing a protein having salutaridinol 7-O-acetyltransferase activity, said method comprising
i) transforming or transfecting a cell with a nucleic acid according to claim 1 , under conditions permitting the expression of the protein having salutaridinol 7-O-acetyltransferase activity,
ii) propagating said cells, and
iii) recovering the thus-produced protein having salutaridinol 7-O-acetyltransferase activity.
5. A method for producing a protein having salutaridinol 7-O-acetyltransferase activity, said method comprising
i) transforming or transfecting a cell with a nucleic acid according to claim 2 , under conditions permitting the expression of the protein having salutaridinol 7-O-acetyltransferase activity,
ii) propagating said cells, and
iii) recovering the thus-produced protein having salutaridinol 7-O-acetyltransferase activity.
6. A method for producing a protein having salutaridinol 7-O-acetyltransferase activity, said method comprising
i) transforming or transfecting a cell with a nucleic acid according to claim 3 , under conditions permitting the expression of the protein having salutaridinol 7-O-acetyltransferase activity,
ii) propagating said cells, and
iii) recovering the thus-produced protein having salutaridinol 7-O-acetyltransferase activity.