Wound repair dressings and methods for their preservation
View Patent ↗Methods and products relating the preservation of cultured mammalian epithelial or mesenchymal cells are provided. The methods involve the pre-treatment of epithelial cells with a solution containing a cryoprotectant amount of monosaccharides and/or disaccharides. The treated cells then are frozen, dried or freeze-dried in a minimum volume of solution and stored for later use. The invention avoids the use of materials that must be washed away from the preserved tissue prior to application of the tissue to a wound bed. The invention also permits the storage of epithelial tissue in a dry state.
1. A method for preserving cultured mammalian epithelial or mesenchymal cells, comprising:
incubating the cells in a cryopreservant solution,
removing excess cryopreservant solution from the cells to form a minimum volume of solution in contact with the cells, wherein said minimum volume is not more than 30 ml solution per 50 cm 2 graft of cultured mammalian epithelial or mesenchymal cells, and
freezing or freeze-drying the cells in contact with the minimum volume of cryoprotectant solution.
2. The method of claim 1 wherein the excess cryopreservant solution is removed by draining.
3. The method of claim 1 wherein the excess cryopreservant solution is removed by aspiration.
4. The method of claim 1 wherein the cells in contact with the minimum volume are frozen.
5. The method of claim 1 wherein the cells in contact with the minimum volume are freeze-dried.
6. The method of claim 1 wherein the cryoprotectant solution contains a cryoprotectant amount of a monosaccharide.
7. The method of claim 1 wherein the cryoprotectant solution contains a cryoprotectant amount of a disaccharide.
8. The method of claim 1 wherein the cryoprotectant solution contains a cryoprotectant amount of glucose.
9. The method of claim 1 wherein the cryoprotectant amount of glucose is between 0.05 and 3.5 M glucose.
10. The method of claim 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 or 9 wherein the cells are a sheet of cultured epithelial cells.
11. The method of claim 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 or 9 wherein the cells are cultured on a substratum.