Functional surface coating
Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group's activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.
1. A method of preparing a functional surface, said method comprising:
providing a substrate;
providing an effective amount of active component comprising a functional group, spacer group, and a binding group;
providing an effective amount of cross-linking component;
providing an effective amount of matrix-forming component;
affixing said active component, said cross-linking component and said matrix forming component onto said substrate, thereby forming a functional surface.
2. The method of claim 1 , wherein said affixing further comprises coating said active component, said cross-linking component and said matrix forming component onto said substrate creating a coated substrate and curing said coated substrate.
3. The method of claim 2 , wherein said curing is thermal activation.
4. The method of claim 1 , wherein said functional group is biotin.
5. The method of claim 1 , wherein said spacer group is polyethylene glycol.
6. The method of claim 1 , wherein said binding group is a silanes.
7. The method of claim 1 , wherein said binding group is an alkoxysilane.
8. The method of claim 1 , wherein said cross-linking component is acrylates.
9. The method of claim 1 , wherein said matrix-forming component is a polyoxyethylene-based surface-active substances.
10. The method of claim 1 , wherein said matrix-forming component is polyoxyethylene sorbitan tetraoleate.