Method and instrument for detecting biomolecular interactions
View Patent ↗Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An instrument system for detecting a biochemical interaction on a biosensor. The system includes an array of detection locations comprises a light source for generating collimated white light. A beam splitter directs the collimated white light towards a surface of a sensor corresponding to the detector locations. A detection system includes an imaging spectrometer receiving the reflected light and generating an image of the reflected light.
1. An instrument system for detecting a biochemical interaction on a biosensor comprising an array of detection locations, said system comprising:
a light source for generating collimated white light,
a beam splitter directing said collimated white light towards a surface of a sensor corresponding to said detector locations; and
a detection system including an imaging spectrometer receiving a reflected light from said sensor and generating an image of said reflected light.
2. The invention of claim 1 wherein said biosensor is a microarray chip.
3. The invention of claim 2 wherein said microarray chip is a conventional microarray chip.
4. The invention of claim 1 wherein said biosensor is transported along a scan direction.
5. The invention of claim 1 wherein said imaging spectrometer generates a Peak Wavelength Value image.
6. The invention of claim 1 wherein said directed collimated white light is directed to a plurality of locations on said surface of said sensor.
7. The invention of claim 1 wherein said directed collimated white light is simultaneously directed to a plurality or locations on said surface of said sensor.
8. The invention of claim 1 wherein said directed collimated white light is directed to an imaging area on said surface of said sensor.
9. The invention of claim 2 wherein said directed collimated white light illuminates a plurality of wells of said microarray chip.
10. The invention of claim 1 wherein said imaging spectrometer includes a two-dimensional Charge Coupled Device camera.
11. The invention of claim 1 wherein said imaging spectrometer includes a diffraction grating.
12. The invention of claim 1 including a software interface, said software interface controlling said imaging spectrometer.
13. The invention of claim 12 wherein said software interface coordinates Peak Wavelength Value determination with an x-y motion stage.
14. The invention of claim 12 wherein said software interface converts measured data into a Peak Wavelength Value.
15. The invention of claim 1 , wherein said light source illuminates said biosensor from a sensor top surface.
16. The invention of claim 1 wherein said light source illuminates said biosensor from a sensor bottom surface.