Alteration of oil traits in plants
View Patent ↗The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
1. A method for altering oil phenotype in a plant which comprises:
(a) transforming a plant with a recombinant DNA construct comprising an isolated nucleotide sequence encoding a plant Hap3/Lec1 transcription factor comprising SEQ ID 134, wherein said isolated nucleic acid sequence is operably linked to at least one regulatory sequence;
(b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
(c) selecting transformed plants having an altered oil phenotype as compared to the oil phenotype of an untransformed plant.
2. A method for altering oil phenotype in a plant which comprises:
(a) transforming a plant with a recombinant DNA construct comprising an isolated nucleotide fragment encoding a plant Hap3/lec1 transcription factor having at least 80% sequence identity based on the Clustal method of alignment when compared to the amino acid sequence set forth in SEQ ID NO:134; wherein said nucleic acid sequence is operably linked to at least one regulatory sequence,
(b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
(c) selecting transformed plants having an altered oil phenotype as compared to the oil phenotype of an untransformed plant.
3. The method of claim 1 or 2 wherein the plant is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica , sorghum, sunflower, and coconut.